Smith Christopher, Santorelli Adam, Engelmann Shaun, Dunn Andrew K
Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA.
Biomed Opt Express. 2023 Jan 17;14(2):771-782. doi: 10.1364/BOE.476178. eCollection 2023 Feb 1.
Monitoring blood flow is critical to treatment efficacy in many surgical settings. Laser speckle contrast imaging (LSCI) is a simple, real-time, label-free optical technique for monitoring blood flow that has emerged as a promising technique but lacks the ability to make repeatable quantitative measurements. Multi-exposure speckle imaging (MESI) is an extension of LSCI that requires increased complexity of instrumentation, which has limited its adoption. In this paper, we design and fabricate a compact, fiber-coupled MESI illumination system (FCMESI) that is substantially smaller and less complex than previous systems. Using microfluidics flow phantoms, we demonstrate that the FCMESI system measures flow with an accuracy and repeatability equivalent to traditional free space MESI illumination systems. With an stroke model, we also demonstrate the ability of FCMESI to monitor cerebral blood flow changes.
在许多手术场景中，监测血流对治疗效果至关重要。激光散斑对比成像（LSCI）是一种简单、实时、无需标记的光学血流监测技术，它已成为一种很有前景的技术，但缺乏进行可重复定量测量的能力。多曝光散斑成像（MESI）是LSCI的一种扩展，其仪器设备更为复杂，这限制了它的应用。在本文中，我们设计并制造了一种紧凑的光纤耦合MESI照明系统（FCMESI），该系统比以前的系统体积更小、复杂度更低。通过微流体流动模型，我们证明FCMESI系统测量血流的准确性和可重复性与传统的自由空间MESI照明系统相当。利用中风模型，我们还展示了FCMESI监测脑血流变化的能力。
