Ogawa H, Sakurami T, Imura H
Nihon Naibunpi Gakkai Zasshi. 1980 Feb 20;56(2):157-63. doi: 10.1507/endocrine1927.56.2_157.
Human peripheral blood lymphocytes were studied with the use of fluorescinated human thyroglobulin (FITC-Tg) for the presence of cells which bind human FITC-Tg (Tg-BL) and which secrete a thyroglobulin antibody (Tg-SL) by culturing them for 5 days with pokeweed mitogen. In seven out of fifteen (47%) patients with Hashimoto's thyroiditis, an average of 0.18% of lymphocytes were able to bind FITC-Tg, while only one out of six (17%) patients with Graves' disease was able to bind them. No Tg-BLs were detected in five patients with miscellaneous autoimmune diseases and five normal subjects. Tg-BLs were identified as B lymphocytes by their inability to make rosettes with sheep red blood cells. Tg-SLs were detected in six out of eight (75%) patients with Hashimoto's thyroiditis and two out of five (40%) patients with Graves' disease. The positive rates of Tg-BL and Tg-SL were fairly well correlated with the thyroglobulin antibody titers.
利用荧光素标记的人甲状腺球蛋白（FITC-Tg）对人外周血淋巴细胞进行研究，通过用商陆有丝分裂原培养5天，检测能够结合人FITC-Tg的细胞（Tg-BL）以及分泌甲状腺球蛋白抗体的细胞（Tg-SL）的存在情况。在15例桥本甲状腺炎患者中的7例（47%），平均0.18%的淋巴细胞能够结合FITC-Tg，而6例格雷夫斯病患者中只有1例（17%）能够结合FITC-Tg。在5例其他自身免疫性疾病患者和5名正常受试者中未检测到Tg-BL。通过不能与绵羊红细胞形成玫瑰花结，将Tg-BL鉴定为B淋巴细胞。在8例桥本甲状腺炎患者中的6例（75%）以及5例格雷夫斯病患者中的2例（40%）检测到Tg-SL。Tg-BL和Tg-SL的阳性率与甲状腺球蛋白抗体滴度有较好的相关性。
