Hunan Key Laboratory for Conservation and Utilization of Biological Resources in the Nanyue Mountainous Region, College of Life Sciences and Environment, Hengyang Normal University, Hengyang, Hunan, China.
Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.
PeerJ. 2023 Feb 28;11:e14773. doi: 10.7717/peerj.14773. eCollection 2023.
L. is one of the large genera in the Apiaceae family. In a previous study, the molecular phylogenies of were explored using nuclear ribosomal DNA internal transcribed spacers (ITS) and several chloroplast DNA segments. There have been few studies conducted on chloroplast genomes in , which has limited systematic understanding of this genus. We assembled the complete chloroplast genomes of nine species from China using data generated from next generation sequencing (NGS). The chloroplast (cp) DNA used were standard double-stranded molecules, ranging from 146,432 base pairs (bp) () to 165,666 bp () in length. The circular DNA contained a large single-copy (LSC) region, small single-copy (SSC) region, and pair of inverted repeats (IRs). The cp DNA of the nine species contained 82-93 protein-coding genes, 36-37 transfer RNA (tRNA) genes, and eight ribosomal RNA (rRNA) genes, respectively. Four species (, , , and ) exhibited striking distinctions in genome size, gene number, IR boundary, and sequence identity. We confirmed the non-monophyly of the species on the basis of the nine newly identified plastomes. The distant relationship between the above-mentioned four species and Pimpinelleae was indicated with high support values. Our study provides a foundation for future in-depth phylogenetic and taxonomic studies of genus .
L. 是伞形科的一个大属。在之前的研究中,使用核核糖体 DNA 内部转录间隔区 (ITS) 和几个叶绿体 DNA 片段对 进行了分子系统发育分析。关于 属的叶绿体基因组的研究很少,这限制了对该属的系统理解。我们使用下一代测序 (NGS) 生成的数据,组装了来自中国的 9 种 属植物的完整叶绿体基因组。所使用的叶绿体 (cp) DNA 是标准的双链分子,长度从 146432 个碱基对 (bp) ()到 165666 bp ()。圆形 DNA 包含一个大单拷贝 (LSC) 区、一个小单拷贝 (SSC) 区和一对反向重复 (IR)。这 9 种 cp DNA 分别包含 82-93 个蛋白编码基因、36-37 个转移 RNA (tRNA) 基因和 8 个核糖体 RNA (rRNA) 基因。四个物种 (、、、和 ) 在基因组大小、基因数量、IR 边界和序列同一性方面表现出显著差异。我们基于这 9 个新鉴定的质体基因组,证实了 属的非单系性。上述四个 属物种与 Pimpinelleae 的亲缘关系较远,支持度很高。我们的研究为今后深入研究属的系统发育和分类学提供了基础。