和氏蜂蜜果冻对ORL115和ORL188细胞的抗氧化活性及凋亡性细胞死亡的诱导作用

The Antioxidant Activity and Induction of Apoptotic Cell Death by and sp. Honey Jelly in ORL115 and ORL188 Cells.

作者信息

Anuar Mohd Nur Nasyriq, Ibrahim Muhammad, Zakaria Nor Hafizah, Ichwan Solachuddin Jauhari Arief, Md Isa Muhammad Lokman, Mat Alewi Nur Aizura, Hagar Abdullah, Abdul Majid Fadzilah Adibah

机构信息

Department of Nutrition Sciences, Kulliyyah of Allied Health Sciences, International Islamic University Malaysia, Pahang, Malaysia.

Institute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia.

出版信息

Malays J Med Sci. 2023 Feb;30(1):82-91. doi: 10.21315/mjms2023.30.1.7. Epub 2023 Feb 28.

DOI:10.21315/mjms2023.30.1.7

PMID:36875196

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9984108/

Abstract

BACKGROUND

Head and neck cancer patients usually need nutritional support due to difficulties in swallowing and chewing. Therefore, this study aimed to formulate and sp. honey jelly (MTJ) as a convenient functional food.

METHODS

The antioxidant properties were analysed using 2,2'-diphenyl-1 picrylhydrazyl (DPPH), ferric reducing antioxidant potential (FRAP) and 2,2'-azinodi 3-ethylbenthiazolinesulfonate (ABTS) assays. Cytotoxicity was assayed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test and the induction of apoptosis was observed via caspase-3/7 activity assay. The identification of phenolic compounds was done via ultra-high-performance-liquid chromatography coupled to mass spectrometer (UHPLC-MS/MS).

RESULTS

The antioxidant analysis exhibited: the half inhibitory concentration (IC) of DPPH inhibition, 54.10 (SD = 4.51) μg/mL; the FRAP value, 30.07 (SD = 0.93) mM TEQ/100 g; and the ABTS value, 131.79 (SD = 8.73) mg TEQ/100 g. Cinnamic acid was the most abundant phenolic compound, followed by maleic acid and salicylic acid. The IC for ORL115 and ORL188 were 35.51 mg/mL and 43.54 mg/mL, respectively. The cells became rounded and dissymmetrical which reduced in number and size. The apoptotic cell death in ORL115 and ORL188 was deduced as caspase-3/7 activities that significantly increased ( < 0.05).

CONCLUSION

The study evidenced that the antioxidant activity of MTJ could influence the induction of apoptosis in ORL115 and ORL188 in future investigations and verifications.

摘要

背景

头颈癌患者通常因吞咽和咀嚼困难而需要营养支持。因此，本研究旨在研制一种方便的功能性食品——刺槐蜂蜜果冻（MTJ）。

方法

采用2,2'-二苯基-1-苦基肼（DPPH）、铁还原抗氧化能力（FRAP）和2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）法分析其抗氧化性能。采用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）试验检测细胞毒性，并通过半胱天冬酶-3/7活性测定观察细胞凋亡诱导情况。通过超高效液相色谱-质谱联用仪（UHPLC-MS/MS）鉴定酚类化合物。

结果

抗氧化分析显示：DPPH抑制的半数抑制浓度（IC）为54.10（标准差=4.51）μg/mL；FRAP值为30.07（标准差=0.93）mM TEQ/100 g；ABTS值为131.79（标准差=8.73）mg TEQ/100 g。肉桂酸是含量最丰富的酚类化合物，其次是马来酸和水杨酸。ORL115和ORL188的IC分别为35.51 mg/mL和43.54 mg/mL。细胞变得圆钝且不对称，数量和大小减少。ORL115和ORL188中的凋亡细胞死亡被推断为半胱天冬酶-3/7活性显著增加（P<0.05）。