Mathiou Vasiliki, Tsiambas Evangelos, Maipas Sotirios, Thymara Irene, Peschos Dimitrios, Lazaris Andreas C, Kavantzas Nikolaos
Mathiou Dental Clinic, Athens, Greece.
Department of Cytology, 417 Veterans Army Hospital (NIMTS), Athens, Greece.
Cancer Diagn Progn. 2023 Mar 3;3(2):189-193. doi: 10.21873/cdp.10200. eCollection 2023 Mar-Apr.
BACKGROUND/AIM: Odontogenic cysts belong to a type of lesions with endodontic origin that in some cases mimic even aggressive odontogenic tumors sharing with them similar radiographic features. Periapical cysts (PCs) belong to the inflammatory odontogenic cysts sub-category and rarely squamous cell carcinoma arises from their hyperplastic/ dysplastic epithelia. This study aimed to explore the impact of cluster differentiation 34 (CD34) protein expression combined with micro vessel density (MVD) on PCs.
Forty-eight (n=48) archival, formalin-fixed, and paraffin-embedded PC tissue specimens were included in the study. Immunohistochemistry (IHC) was performed in the corresponding tissue sections using an anti- CD34 antibody. CD34 expression levels and also MVD in the examined cases were measured by implementing a digital image analysis protocol.
CD34 over-expression (moderate to high staining intensity levels) were detected in 29/48 (60.4%) cases, whereas the rest of them (19/48-39.6%) were characterized by low levels of expression. Extended MVD was identified in 26/48 (50.1%) cases correlated with CD34 over-expression, epithelial hyperplasia (p-value=0.001), and marginally with inflammatory infiltration level in the examined lesions (p-value=0.056).
CD34 over-expression combined with increased MVD is associated with a neoplastic-like (hyperplastic) phenotype in PCs as a result of increased neo-angiogenic activity. These histopathological characteristics rarely form an eligible substrate for squamous cell carcinoma onset in untended cases.
背景/目的：牙源性囊肿属于一类起源于牙髓的病变，在某些情况下，其影像学特征与侵袭性牙源性肿瘤相似。根尖囊肿（PCs）属于炎症性牙源性囊肿亚类，其增生/发育异常上皮很少发生鳞状细胞癌。本研究旨在探讨34簇分化抗原（CD34）蛋白表达联合微血管密度（MVD）对根尖囊肿的影响。
本研究纳入48例存档的、经福尔马林固定和石蜡包埋的根尖囊肿组织标本。使用抗CD34抗体在相应组织切片上进行免疫组织化学（IHC）检测。通过数字图像分析方案测量所检查病例中的CD34表达水平和MVD。
在29/48（60.4%）例病例中检测到CD34过表达（中等至高染色强度水平），其余病例（19/48 - 39.6%）表现为低表达水平。在26/48（50.1%）例病例中发现MVD增加，与CD34过表达、上皮增生相关（p值 = 0.001），与所检查病变中的炎症浸润水平有轻微相关性（p值 = 0.056）。
CD34过表达联合MVD增加与根尖囊肿中肿瘤样（增生性）表型相关，这是由于新生血管生成活性增加所致。在未处理的病例中，这些组织病理学特征很少形成鳞状细胞癌发生的合适底物。
