Feng Liang, Chen Hua, Zhao Jun, Liu Dan, Wei Zhen, Li Yuchan, Yang Bin, He Yilian, Zhang Meng, Hou Dabin, Yu Ma
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China.
Institute of Biomass Energy, Neijiang Academy of Agricultural Sciences of Sichuan Province, Neijiang 641000, China.
Chin Herb Med. 2022 May 23;15(1):57-62. doi: 10.1016/j.chmed.2022.03.006. eCollection 2023 Jan.
The content of saikosaponins in genus is increased with numbers of lateral root, but the genetic mechanisms are largely unknown. This study aims to identify the heme oxygenase (HO) gene family members of and , and assess their role in the root development in .
The gene sequences of HO family were selected from -seq full-length transcriptome data of and , and were analyzed in physicochemical properties, conserved domains, motifs and phylogenetic relationship. In addition, the expression patterns of HO gene in different parts of roots were compared via transcriptome sequencing and qRT-PCR in the two species.
Five HO genes (-) belonging to the HO1 subfamily were identified from the transcriptome data, whereas the HO2 subfamily member was not identified. The expression levels of and were significantly higher than those of other three HO members in the transcriptome analysis. In addition, the expression profile of showed consistency with lateral root development in and .
Hos might participate in the auxin-induced morphogenesis of lateral roots. The yield of saikosaponin may be improved by manipulating expression of these genes.
柴胡属植物中柴胡皂苷的含量随侧根数量增加而升高，但其遗传机制尚不清楚。本研究旨在鉴定柴胡属植物柴胡（Bupleurum chinense）和狭叶柴胡（Bupleurum scorzonerifolium）的血红素加氧酶（HO）基因家族成员，并评估其在柴胡属植物根系发育中的作用。
从柴胡和狭叶柴胡的RNA-seq全长转录组数据中筛选HO家族的基因序列，并对其理化性质、保守结构域、基序和系统发育关系进行分析。此外，通过转录组测序和qRT-PCR比较HO基因在两个物种不同根段中的表达模式。
从转录组数据中鉴定出5个属于HO1亚家族的柴胡HO基因（BcHO1-BcHO5），未鉴定出HO2亚家族成员。转录组分析显示，BcHO1和BcHO2的表达水平显著高于其他3个HO成员。此外，BcHO1的表达谱与柴胡和狭叶柴胡侧根发育一致。
HOs可能参与生长素诱导的侧根形态建成。通过调控这些基因的表达可能提高柴胡皂苷的产量。
