柴胡皂苷生物合成相关基因在柴胡（Bupleurum chinense DC.）和狭叶柴胡（Bupleurum scorzonerifolium Willd.）之间的差异表达
Differential Expression of Genes Involved in Saikosaponin Biosynthesis Between DC. and Willd.
作者信息
Yu Ma, Chen Hua, Liu Shi-Hang, Li Yu-Chan, Sui Chun, Hou Da-Bin, Wei Jian-He
机构信息
Institute of Medicinal Plant Development (IMPLAD), Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
School of Life Sciences and Engineering, Southwest University of Science and Technology, Mianyang, China.
出版信息
Front Genet. 2020 Oct 16;11:583245. doi: 10.3389/fgene.2020.583245. eCollection 2020.
Radix Bupleuri (roots of spp.) is an important medicinal herb. Triterpenoid saponins of saikosaponins generally constitute the main class of secondary metabolites of plants in the genus. However, the molecular regulatory mechanism underlying their biosynthesis remains elusive. In this study, we observed significantly different saikosaponin biosynthesis between and at the seedling stage. The sequential and expression characterization of 232 genes in the triterpenoid saponin biosynthetic pathway, which includes the mevalonate (MVA) pathway and methylerythritol phosphate (MEP) pathway, between and was also investigated. Sixty of these genes may be involved in saikosaponin biosynthesis. Manipulation of these genes, especially those of the β-AS, P450, and UGT families, may improve saikosaponin production.
柴胡（柴胡属植物的根）是一种重要的药用植物。柴胡皂苷类三萜皂苷通常构成该属植物次生代谢产物的主要类别。然而，其生物合成的分子调控机制仍不清楚。在本研究中，我们观察到[具体两种柴胡品种名称未给出]和[具体两种柴胡品种名称未给出]在幼苗期柴胡皂苷生物合成存在显著差异。还研究了[具体两种柴胡品种名称未给出]和[具体两种柴胡品种名称未给出]之间三萜皂苷生物合成途径中232个基因的序列和表达特征，该途径包括甲羟戊酸（MVA）途径和甲基赤藓糖醇磷酸（MEP）途径。其中60个基因可能参与柴胡皂苷的生物合成。对这些基因进行调控，尤其是β-AS、P450和UGT家族的基因，可能会提高柴胡皂苷的产量。
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