Liu Jianguang, Zhao Guiyuan, Geng Jinpeng, Geng Zhao, Dou Haikuan, Liu Xu, An Zetong, Zhang Hanshuang, Wang Yongqiang
Institute of Cotton, Hebei Academy of Agriculture and Forestry Sciences/Key Laboratory of Biology and Genetic Improvement of Cotton in Huanghuaihai Semiarid Area, Shijiazhuang, China.
School of Science, Hebei University of Technology, Tianjin, China.
Front Plant Sci. 2023 Feb 16;14:1056662. doi: 10.3389/fpls.2023.1056662. eCollection 2023.
Carbon ion beam (CIB) irradiation is a powerful way to create mutations in animals, plants, and microbes. Research on the mutagenic effects and molecular mechanisms of radiation is an important and multidisciplinary issue. However, the effect of carbon ion radiation on cotton is uncertain. In this study, five different upland cotton varieties and five CIB doses were used to identify the suitable irradiation dose for cotton. Three mutagenized progeny cotton lines from the wild-type Ji172 were re-sequenced. The effect of half-lethal dose on mutation induction indicated that 200 Gy with LET of 226.9 KeV/μm was the most effective heavy-ion dose for upland cotton and a total of 2,959-4,049 single-base substitutions (SBSs) and 610-947 insertion-deletion polymorphisms (InDels) were identified among the three mutants by resequencing. The ratio of transition to transversion in the three mutants ranged from 2.16 to 2.24. Among transversion events, G:C>C:G was significantly less common than three other types of mutations (A:T>C:G, A:T>T:A, and G:C>T:A). The proportions of six types of mutations were very similar in each mutant. The distributions of identified SBSs and InDels were similar with unevenly distributed across the genome and chromosomes. Some chromosomes had significantly more SBSs than others, and there were "hotspot" mutation regions at the ends of chromosomes. Overall, our study revealed a profile of cotton mutations caused by CIB irradiation, and these data could provide valuable information for cotton mutation breeding.
碳离子束（CIB）辐照是在动物、植物和微生物中产生突变的一种有效方法。对辐射诱变效应和分子机制的研究是一个重要的多学科问题。然而，碳离子辐射对棉花的影响尚不确定。在本研究中，使用了五个不同的陆地棉品种和五种CIB剂量来确定棉花的适宜辐照剂量。对来自野生型冀172的三个诱变后代棉花品系进行了重测序。半致死剂量对诱变的影响表明，线能量转移（LET）为226.9 keV/μm的200 Gy是陆地棉最有效的重离子剂量，通过重测序在三个突变体中总共鉴定出2959 - 4049个单碱基替换（SBSs）和610 - 947个插入缺失多态性（InDels）。三个突变体中转换与颠换的比例在2.16至2.24之间。在颠换事件中，G:C>C:G明显不如其他三种类型的突变（A:T>C:G、A:T>T:A和G:C>T:A）常见。六种类型的突变在每个突变体中的比例非常相似。鉴定出的SBSs和InDels的分布相似，在基因组和染色体上分布不均。一些染色体的SBSs明显多于其他染色体，并且在染色体末端存在“热点”突变区域。总体而言，我们的研究揭示了CIB辐照引起的棉花突变概况，这些数据可为棉花突变育种提供有价值的信息。
