Afshar Akbar Safipour, Abbaspour Hossein
Department of Biology, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran.
Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran.
Physiol Mol Biol Plants. 2023 Feb;29(2):263-276. doi: 10.1007/s12298-023-01279-8. Epub 2023 Jan 23.
This study investigated how inoculation of salt-stressed seedlings with , an arbuscular mycorrhizal fungus (AMF), affects their biomass, oxidative damage, antioxidant enzyme activity, and gene expression. Pistachio seedlings (N:36) were randomly assigned to AMF inoculation and non-inoculation groups in a pot experiment with 9 replications. Each group was further divided and randomly assigned to two salinity treatments (0 and 300 mM NaCl). At the end of week 4, three pistachio plantlets were randomly selected from each group for colonization inspection, physiological and biochemical assays, and biomass measurements. Salinity activated enzymatic and non-enzymatic antioxidant systems in the pistachio plants were studied. The negative effects of salinity included reduced biomass and relative water content (RWC), increased O , HO, MDA, and electrolytic leakage. Generally, was found to mitigate the adverse effects of salinity in pistachio seedlings. AMF inoculation resulted in even further increases in the activities of SODs, POD, CAT, and GR enzymes, upregulating Cu/Zn-SOD, Fe-SOD, Mn-SOD, and GR genes expression in plants under salinity stress. Moreover, AMF significantly increased AsA, α-tocopherol, and carotenoids under both control and salinity conditions. The study concludes with a call for future research into the mechanisms of mycorrhiza-induced tolerance in plants under salinity stress.
The online version contains supplementary material available at 10.1007/s12298-023-01279-8.
本研究调查了用丛枝菌根真菌（AMF）接种盐胁迫幼苗如何影响其生物量、氧化损伤、抗氧化酶活性和基因表达。在一个盆栽试验中，将36株阿月浑子幼苗随机分为AMF接种组和未接种组，每组9次重复。每组再进一步划分并随机分配到两种盐度处理（0和300 mM NaCl）。在第4周结束时，从每组中随机选择3株阿月浑子幼苗进行菌根定殖检查、生理生化测定和生物量测量。研究了盐度激活的阿月浑子植物酶促和非酶促抗氧化系统。盐度的负面影响包括生物量和相对含水量（RWC）降低，O₂、H₂O₂、丙二醛（MDA）和电解质渗漏增加。一般来说，发现AMF可减轻盐度对阿月浑子幼苗的不利影响。AMF接种导致超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）和谷胱甘肽还原酶（GR）的活性进一步增加，上调了盐胁迫下植物中铜/锌超氧化物歧化酶（Cu/Zn-SOD）、铁超氧化物歧化酶（Fe-SOD）、锰超氧化物歧化酶（Mn-SOD）和GR基因的表达。此外，在对照和盐度条件下，AMF均显著增加了抗坏血酸（AsA）、α-生育酚和类胡萝卜素的含量。该研究呼吁未来对盐胁迫下菌根诱导植物耐受性的机制进行研究。
在线版本包含可在10.1007/s12298-023-01279-8获取的补充材料。
