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异硫氰酸苄酯通过降低真菌载量和抑制Mincle信号通路改善角膜炎的预后。

Benzyl isothiocyanate improves the prognosis of keratitis by reducing fungal load and inhibiting Mincle signal pathway.

作者信息

Yi Wendan, Gu Lingwen, Wang Yuwei, Lin Jing, Zhang Lina, Wang Qian, Diao Weilin, Qi Yinghe, Chi Menghui, Yin Min, Li Cui, Zhao Guiqiu

机构信息

Department of Ophthalmology, The Affiliated Hospital of Qingdao University, Qingdao, China.

出版信息

Front Microbiol. 2023 Feb 16;14:1119568. doi: 10.3389/fmicb.2023.1119568. eCollection 2023.

DOI:10.3389/fmicb.2023.1119568
PMID:36876115
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9978348/
Abstract

keratitis is a potential blinding disease associated with invasion and excessive inflammatory response. Benzyl isothiocyanate (BITC) is a secondary metabolite with broad antibacterial and anti-inflammatory activity extracted from cruciferous species. However, the role of BITC in keratitis has not been discovered yet. This study aims to explore the antifungal and anti-inflammatory effects and mechanisms of BITC in keratitis. Our results provided evidences that BITC exerted antifungal effects against by damaging cell membranes, mitochondria, adhesion, and biofilms in a concentration-dependent manner. , fungal load and inflammatory response including inflammatory cell infiltration and pro-inflammatory cytokine expression were reduced in BITC-treated keratitis. Additionally, BITC significantly decreased Mincle, IL-1β, TNF-α, and IL-6 expression in RAW264.7 cells that stimulated by or Mincle ligand trehalose-6,6-dibehenate. In summary, BITC possessed fungicidal activities and could improve the prognosis of keratitis by reducing fungal load and inhibiting the inflammatory response mediated by Mincle.

摘要

角膜炎是一种与侵袭和过度炎症反应相关的潜在致盲性疾病。异硫氰酸苄酯（BITC）是一种从十字花科植物中提取的具有广泛抗菌和抗炎活性的次生代谢产物。然而，BITC在角膜炎中的作用尚未被发现。本研究旨在探讨BITC在角膜炎中的抗真菌和抗炎作用及机制。我们的结果表明，BITC通过以浓度依赖的方式破坏细胞膜、线粒体、黏附及生物膜，对[具体真菌名称未给出]发挥抗真菌作用。此外，在BITC治疗的角膜炎中，真菌负荷以及包括炎症细胞浸润和促炎细胞因子表达在内的炎症反应均有所降低。此外，BITC显著降低了由[具体刺激物未给出]或Mincle配体海藻糖-6,6-二山嵛酸酯刺激的RAW264.7细胞中Mincle、IL-1β、TNF-α和IL-6的表达。总之，BITC具有杀菌活性，可通过降低真菌负荷和抑制由Mincle介导的炎症反应来改善角膜炎的预后。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5e/9978348/9284a4de9563/fmicb-14-1119568-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5e/9978348/a8e118b8332f/fmicb-14-1119568-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5e/9978348/36e89be969de/fmicb-14-1119568-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5e/9978348/520470438fb2/fmicb-14-1119568-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5e/9978348/051635be9924/fmicb-14-1119568-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5e/9978348/f116715c3d00/fmicb-14-1119568-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5e/9978348/dc55b5b66bcf/fmicb-14-1119568-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5e/9978348/4acb2a294963/fmicb-14-1119568-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5e/9978348/9284a4de9563/fmicb-14-1119568-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5e/9978348/a8e118b8332f/fmicb-14-1119568-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5e/9978348/36e89be969de/fmicb-14-1119568-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5e/9978348/520470438fb2/fmicb-14-1119568-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5e/9978348/051635be9924/fmicb-14-1119568-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5e/9978348/f116715c3d00/fmicb-14-1119568-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5e/9978348/dc55b5b66bcf/fmicb-14-1119568-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5e/9978348/4acb2a294963/fmicb-14-1119568-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5e/9978348/9284a4de9563/fmicb-14-1119568-g008.jpg

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