Department of Food Energy System Science, Graduate School of Bio-Application and System Engineering Sciences, Tokyo University of Agriculture and Technology, 2-24-16, Tokyo 184-8588, Japan.
Institute of Food Research, National Agriculture and Food Research Organization (NARO), 2-1-12 Kannondai, Tsukuba 305-8642, Japan.
Toxins (Basel). 2022 Nov 2;14(11):756. doi: 10.3390/toxins14110756.
Aflatoxins (AFs), a class of toxins produced by certain species of the genus , occasionally contaminate food and cause serious damage to human health and the economy. AFs contamination is a global problem, and there is a need to develop effective strategies to control aflatoxigenic fungi. In this study, we focused on isothiocyanates (ITCs) as potential chemical agents for the control of aflatoxigenic fungi. We quantitatively evaluated the effects of four ITCs (allyl ITC (AITC), benzyl ITC (BITC), and methyl and phenylethyl ITCs) in dissolved and gaseous states on the growth and aflatoxin B production of . In experiments using dissolved ITCs, BITC was found to be the strongest inhibitor of growth and aflatoxin B production by . Meanwhile, in the gaseous state, AITC strongly inhibited the growth. When the concentration of ITCs in the liquid medium was quantified over time, AITC levels decreased to below the detection limit within 24 h, whereas BITC levels remained stable even after 48 h. These results suggested that when ITCs are utilized to control aflatoxigenic fungi, it is necessary to use them in a dissolved or gaseous state, depending on their volatility.
黄曲霉毒素（AFs）是某些种类的真菌产生的一类毒素，偶尔会污染食物，对人类健康和经济造成严重损害。AFs 污染是一个全球性问题，因此需要开发有效的策略来控制产黄曲霉毒素真菌。在这项研究中，我们专注于异硫氰酸酯（ITCs）作为控制产黄曲霉毒素真菌的潜在化学试剂。我们定量评估了四种 ITCs（丙烯基异硫氰酸酯（AITC）、苄基异硫氰酸酯（BITC）以及甲基和苯乙基异硫氰酸酯）在溶解和气体状态下对 的生长和黄曲霉毒素 B 产生的影响。在使用溶解 ITCs 的实验中，发现 BITC 是抑制 生长和黄曲霉毒素 B 产生的最强抑制剂。同时，在气体状态下，AITC 强烈抑制 的生长。当随着时间推移定量检测液体培养基中的 ITCs 浓度时，AITC 水平在 24 小时内降至检测限以下，而 BITC 水平即使在 48 小时后仍保持稳定。这些结果表明，当使用 ITCs 来控制产黄曲霉毒素真菌时，需要根据其挥发性将其以溶解或气体状态使用。
