Wang Tiaolan, Li Yongcai, Bi Yang, Zhang Miao, Zhang Tingting, Zheng Xiaoyuan, Dong Yupeng, Huang Yi
College of Food Science and Engineering, Gansu Agricultural University Lanzhou 730070 China
RSC Adv. 2020 Jan 8;10(3):1829-1837. doi: 10.1039/c9ra09225k. eCollection 2020 Jan 7.
The antifungal activity of benzyl isothiocyanate (BITC) against pear pathotype-, the causal agent of pear black spot, and its possible mechanisms were studied. The results indicated that both the spore germination and mycelial growth of were significantly inhibited by BITC in a dose-dependent manner. BITC concentrations at 1.25 mM completely suppressed mycelial growth of and prevented ≥50% of black spot development in wounded pears inoculated with . Microscopic analyses and propidium iodide (PI) staining showed that spore morphology in treated with BITC at 0.625 mM was severely damaged. Relative electrical conductivity and lysis ability assays further showed that BITC treatment destroyed the integrity of the plasma membrane. Additionally, mycotoxin production was inhibited by 0.312 mM BITC, and the inhibitory rates of alternariol monomethyl ether (AME), alternariol (AOH), altenuene (ALT) and tentoxin (TEN) were 89.36%, 84.57%, 91.41% and 67.78%, respectively. The above results suggest that BITC exerts antifungal activity through membrane-targeted mechanisms.
研究了异硫氰酸苄酯（BITC）对梨黑斑病菌梨致病型的抗真菌活性及其可能机制。结果表明，BITC对梨黑斑病菌的孢子萌发和菌丝生长均有显著抑制作用，且呈剂量依赖性。1.25 mM的BITC浓度完全抑制了梨黑斑病菌的菌丝生长，并防止接种该病菌的受伤梨中≥50%的黑斑发展。显微镜分析和碘化丙啶（PI）染色表明，用0.625 mM的BITC处理的梨黑斑病菌孢子形态受到严重破坏。相对电导率和裂解能力测定进一步表明，BITC处理破坏了质膜的完整性。此外，0.312 mM的BITC抑制了霉菌毒素的产生，对单甲基交替霉素（AME）、交替霉素（AOH）、互隔交链孢酚（ALT）和细交链孢菌酮酸（TEN）的抑制率分别为89.36%、84.57%、91.41%和67.78%。上述结果表明，BITC通过膜靶向机制发挥抗真菌活性。
