Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
Histol Histopathol. 2023 Nov;38(11):1257-1267. doi: 10.14670/HH-18-601. Epub 2023 Feb 28.
Different subpopulations of monocytes play roles in phagocytosis, inflammation, and angiogenic processes e.g., Tie2-expressing monocytes (TEMs). The brain is flooded with macrophages that are derived from monocytes within 3-7 days after a stroke. This study aimed to determine the expression level of Tie2 (an angiopoietin receptor) on monocytes and their subpopulations in ischemic stroke patients using the histological and immunohistological study of bone marrow biopsies and blood flow cytometry examination.
Ischemic stroke patients within two days were selected. Participants in the control group were healthy volunteers of matched age and gender. Sample collection was performed within 24 to 48 hours after medical consultants confirmed the stroke diagnosis. An iliac crest bone marrow biopsy was obtained and fixed for histological and immunohistological staining with antiCD14 and antiCD68. Flow cytometry was used to determine the total monocyte population, monocyte subpopulations, and TEMs after staining with monoclonal antibodies to CD45, CD14, CD16, and Tie2.
Post-stroke patients' bone marrow cells were hypercellular. There was an apparent increase in CD68 and CD14-positive cells. Ischemic stroke patients exhibited low percentages of nonclassical monocytes CD14lowCD16++, with an increase in intermediate monocytes CD14highCD16+. Moreover, ischemic stroke patients had significantly higher levels of TEMs than control group.
The results of this study demonstrate dysregulation of angiogenesis in monocyte subsets in ischemic stroke patients, which could be used as an early diagnostic marker of neurovascular damage and may need angiogenic therapy or improved medications to prevent further damage of blood vessels.
不同亚群的单核细胞在吞噬作用、炎症和血管生成过程中发挥作用，例如，Tie2 表达的单核细胞（TEMs）。中风后 3-7 天，大脑中充斥着源自单核细胞的巨噬细胞。本研究旨在通过骨髓活检的组织学和免疫组织化学研究以及血流细胞术检查，确定缺血性中风患者单核细胞及其亚群上 Tie2（血管生成素受体）的表达水平。
选择中风后两天内的患者。对照组的参与者为年龄和性别匹配的健康志愿者。在医疗顾问确认中风诊断后 24 至 48 小时内进行样本采集。采集髂嵴骨髓活检标本，进行组织学和免疫组织化学染色，使用抗 CD14 和抗 CD68。使用针对 CD45、CD14、CD16 和 Tie2 的单克隆抗体对总单核细胞群体、单核细胞亚群和 TEM 进行流式细胞术检测。
中风后患者的骨髓细胞呈高细胞性。CD68 和 CD14 阳性细胞明显增加。缺血性中风患者的非经典单核细胞 CD14lowCD16++百分比较低，中间单核细胞 CD14highCD16+增加。此外，缺血性中风患者的 TEMs 水平明显高于对照组。
本研究结果表明，缺血性中风患者单核细胞亚群的血管生成失调，可作为神经血管损伤的早期诊断标志物，可能需要血管生成治疗或改善药物来防止血管进一步损伤。
