Yang Kexin, Liu Danqing, Teng Runxin, Li Chang, Fan Zhen, Du Jianzhong
Department of Polymeric Materials, School of Materials Science and Engineering, Tongji University, 4800 Caoan Road, Shanghai 201804, China.
Department of Orthopedics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200072, China.
ACS Biomater Sci Eng. 2023 Apr 10;9(4):1900-1908. doi: 10.1021/acsbiomaterials.3c00131. Epub 2023 Mar 6.
Delayed implant-associated infection is an important challenge, as the treatment involves a high risk of implant replacement. Mussel-inspired antimicrobial coatings can be applied to coat a variety of implants in a facile way, but the adhesive 3,4-dihydroxyphenylalanine (DOPA) group is prone to oxidation. Therefore, an antibacterial polypeptide copolymer poly(Phe--Lys)--polyTyr was designed to prepare the implant coating upon tyrosinase-induced enzymatic polymerization for preventing implant-associated infections. Both poly(Phe--Lys) and polyTyr blocks have specific functions: the former provides intrinsic antibacterial activity with a low risk to induce antimicrobial resistance, and the latter is attachable to the surface of implants to rapidly generate an antibacterial coating by injection of polypeptide copolymer since tyrosine could be oxidized to DOPA under catalyzation of skin tyrosinase. This polypeptide coating with excellent antibacterial effect and desirable biofilm inhibition activity is promising for broad applications in a multitude of biomedical materials to combat delayed infections.
延迟性种植体相关感染是一项重大挑战，因为其治疗涉及种植体置换的高风险。受贻贝启发的抗菌涂层可以简便的方式应用于各种种植体涂层，但具有粘性的3,4-二羟基苯丙氨酸（DOPA）基团易于氧化。因此，设计了一种抗菌多肽共聚物聚（苯丙氨酸-赖氨酸）-聚酪氨酸，用于在酪氨酸酶诱导的酶促聚合反应中制备种植体涂层，以预防种植体相关感染。聚（苯丙氨酸-赖氨酸）和聚酪氨酸嵌段均具有特定功能：前者具有内在抗菌活性，诱导抗菌耐药性的风险较低，后者可附着于种植体表面，通过注射多肽共聚物快速生成抗菌涂层，因为酪氨酸在皮肤酪氨酸酶的催化下可氧化为DOPA。这种具有优异抗菌效果和理想生物膜抑制活性的多肽涂层有望广泛应用于多种生物医学材料中，以对抗延迟性感染。
