Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
Biosafety Research Center, Chinese Academy of Agricultural Sciences (CAAS), Shanghai, China.
Virus Genes. 2023 Jun;59(3):417-426. doi: 10.1007/s11262-023-01981-5. Epub 2023 Mar 6.
Bovine gammaherpesvirus 4 (BoHV-4) is a common virus detected in bovine with respiratory disease worldwide. In this study, we identified and characterized a novel BoHV-4 strain, referred as HB-ZJK, in vaginal swabs collected from cattle in China, 2022. The long unique region (LUR) of HB-ZJK is 10,9811 bp in length. It shares 99.17% to 99.38% nucleotide identity to five BoHV-4 strains available in GenBank and the highest similarity was seen with BoHV-4V. test (JN133502.1) strain (99.38%). Mutations, insertions or deletions were observed mainly in HB-ZJK gB (ORF8), TK (ORF21), gH (ORF22), MCP (ORF25), PK (ORF36), gM (ORF39), and gL (ORF47) genes compared to its genomic coordinates. Phylogenetic analyses of gB and TK genes showed that HB-ZJK clustered with China 512 (2019), B6010 (2009), and J4034 (2009) strains, demonstrating that the isolated HB-ZJK belongs to genotype 1. This is the first report that has revealed a comprehensive genome profile of BoHV-4 strain in China. This study will provide foundation for epidemiological investigations of BoHV-4 and contribute to the molecular and pathogenic studies of BoHV-4.
牛疱疹病毒 4 型（BoHV-4）是一种在全球范围内引起牛呼吸道疾病的常见病毒。本研究中，我们从中国牛的阴道拭子中鉴定并鉴定了一种新型 BoHV-4 株，称为 HB-ZJK。HB-ZJK 的长独特区（LUR）长 109811bp。它与 GenBank 中可用的 5 株 BoHV-4 株的核苷酸同一性为 99.17%至 99.38%，与 BoHV-4V 株的相似性最高（99.38%）。测试（JN133502.1）株。HB-ZJK gB（ORF8）、TK（ORF21）、gH（ORF22）、MCP（ORF25）、PK（ORF36）、gM（ORF39）和 gL（ORF47）基因的突变、插入或缺失主要观察到与基因组坐标相比。gB 和 TK 基因的系统进化分析表明，HB-ZJK 与中国 512（2019）、B6010（2009）和 J4034（2009）株聚集在一起，表明分离的 HB-ZJK 属于基因型 1。这是首次报道揭示中国 BoHV-4 株的全面基因组特征。本研究将为 BoHV-4 的流行病学调查提供基础，并为 BoHV-4 的分子和发病机制研究做出贡献。
