Semerci Sevimli Tuğba, Sevimli Murat, Qomi Ekenel Emilia, Altuğ Tasa Burcugül, Nur Soykan Merve, Demir Güçlüer Zilif, İnan Ulukan, Uysal Onur, Güneş Bağış Sibel, Çemrek Fatih, Eker Sarıboyacı Ayla
Department of Stem Cell, Cellular Therapy and Stem Cell Production Application and Research Center (ESTEM), Eskisehir Osmangazi University, 26040 Eskisehir, Turkey; Department of Stem Cell, Institute of Health Sciences, Eskisehir Osmangazi University, 26040 Eskisehir, Turkey.
Department of Histology and Embryology, Faculty of Medicine, Suleyman Demirel University, 32260 Isparta, Turkey.
Gene. 2023 May 20;865:147337. doi: 10.1016/j.gene.2023.147337. Epub 2023 Mar 5.
This study aimed to investigate the differences between the exosomal microRNA-127-5p expression profiles of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) and human synovial fluid-derived mesenchymal stem cells (hSF-MSCs) during chondrogenesis in terms of regenerative treatment of cartilage. Synovial fluid-derived mesenchymal stem cells, adipose tissue-derived mesenchymal stem cells, and human fetal chondroblast cells (hfCCs) were directed to chondrogenic differentiation. Alcian Blue and Safranin O stainings were performed to detect chondrogenic differentiation histochemically. Exosomes derived from chondrogenic differentiated cells and their exosomes were isolated and characterized. microRNA-127-5p expressions were measured by Quantitative reverse transcription PCR (qRT-PCR). Significantly higher levels of microRNA-127-5p expression in differentiated hAT-MSCs exosomes, similar to human fetal chondroblast cells, which are the control group in the chondrogenic differentiation process, were observed. hAT-MSCs are better sources of microRNA-127-5p than hSF-MSCs for stimulating chondrogenesis or in the regenerative therapy of cartilage-related pathologies. hAT-MSCs exosomes are rich sources of microRNA-127-5p and can be an essential candidate for cartilage regeneration treatments.
本研究旨在探讨在软骨再生治疗方面，人脂肪组织来源的间充质干细胞（hAT-MSCs）和人滑液来源的间充质干细胞（hSF-MSCs）在软骨形成过程中外泌体微小RNA-127-5p表达谱的差异。将滑液来源的间充质干细胞、脂肪组织来源的间充质干细胞和人胎儿软骨细胞（hfCCs）诱导进行软骨分化。进行阿尔辛蓝和番红O染色以组织化学检测软骨分化。分离并鉴定来自软骨分化细胞的外泌体及其外泌体。通过定量逆转录聚合酶链反应（qRT-PCR）测量微小RNA-127-5p的表达。观察到，在分化的hAT-MSCs外泌体中，微小RNA-127-5p的表达水平显著高于hSF-MSCs外泌体，类似于软骨分化过程中的对照组人胎儿软骨细胞。在刺激软骨形成或软骨相关疾病的再生治疗中，hAT-MSCs比hSF-MSCs是更好的微小RNA-127-5p来源。hAT-MSCs外泌体是微小RNA-127-5p的丰富来源，可能是软骨再生治疗的重要候选者。
