微小RNA在糖尿病性肌腱病中的作用新见解：脂肪间充质干细胞条件培养基作为一种潜在的基于表观遗传学的肌腱愈合创新疗法
New Insights on the miRNA Role in Diabetic Tendinopathy: Adipose-Derived Mesenchymal Stem Cell Conditioned Medium as a Potential Innovative Epigenetic-Based Therapy for Tendon Healing.
作者信息
Russo Marina, Lepre Caterina Claudia, Conza Gianluca, Tangredi Nicoletta, D'Amico Giovanbattista, Braile Adriano, Moretti Antimo, Tarantino Umberto, Gimigliano Francesca, D'Amico Michele, Trotta Maria Consiglia, Toro Giuseppe
机构信息
Department of Mental, Physical Health and Preventive Medicine, University of Campania "Luigi Vanvitelli", 80138 Naples, Italy.
School of Pharmacology and Clinical Toxicology, University of Campania "Luigi Vanvitelli", 80138 Naples, Italy.
出版信息
Biomolecules. 2025 Feb 11;15(2):264. doi: 10.3390/biom15020264.
BACKGROUND
Adipose-derived mesenchymal stem cell conditioned medium (ASC-CM) improved the viability and wound closure of human tenocytes (HTCN) exposed to high glucose (HG) by activating the transforming growth factor beta 1 (TGF-β1) pathway.
OBJECTIVES
Since ASC-CM can also modulate microRNAs (miRNAs) in recipient cells, this study investigated the effects of ASC-CM on the miRNAs regulating tendon repair (miR-29a-3p, miR-210-3p and miR-21-5p) in HG-HTNC.
METHODS
ASC-CM was obtained by ASCs isolated from the abdominal fat tissue of seven non-diabetic patients. HTNC were cultured in HG for 20 days, then scratched and exposed for 24 h to ASC-CM. qRT-PCR and ELISAs assessed miRNA and target levels.
RESULTS
HG-HTNC exhibited a significant downregulation of miRNAs. ASC-CM restored the levels of miRNAs and their related targets involved in tendon repair.
CONCLUSIONS
The epigenetic modulation observed in HG-HTNC exposed to ASC-CM could be an innovative option in the management of diabetic tendinopathy.
背景
脂肪来源的间充质干细胞条件培养基（ASC-CM）通过激活转化生长因子β1（TGF-β1）途径，提高了暴露于高糖（HG）环境下的人肌腱细胞（HTCN）的活力并促进了伤口愈合。
目的
由于ASC-CM还可以调节受体细胞中的微小RNA（miRNA），因此本研究调查了ASC-CM对高糖环境下HTCN中调节肌腱修复的miRNA（miR-29a-3p、miR-210-3p和miR-21-5p）的影响。
方法
从7名非糖尿病患者的腹部脂肪组织中分离出脂肪干细胞，获取ASC-CM。将HTCN在高糖环境中培养20天，然后划痕并暴露于ASC-CM中24小时。采用qRT-PCR和酶联免疫吸附测定法评估miRNA及其靶标水平。
结果
高糖环境下的HTCN表现出miRNA的显著下调。ASC-CM恢复了参与肌腱修复的miRNA及其相关靶标的水平。
结论
在暴露于ASC-CM的高糖环境下的HTCN中观察到的表观遗传调控可能是糖尿病性肌腱病治疗中的一种创新选择。
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