[Biological function and clinical significance of long non-coding RNA in head and neck squamous carcinoma].

To investigate the relationship between the long-non-coding RNA expression and the clinicopathological parameters of head and neck squamous cell carcinoma (HNSCC) and the biological function of in HNSCC cells. The expression level of in the HNSCC was analyzed using transcriptome sequencing data from TCGA (The Cancer Genome Atlas) database, and the expressions of in laryngeal squamous cell carcinoma tissues (LSCC) of 27 patients in the First Hospital of Shanxi Medical University were detected by transcriptome sequencing. The expression levels of in human embryonic lung diploid cells 2BS, HNSCC cell lines FD-LSC-1, CAL-27 and Detroit562 were determined by real-time quantitative polymerase chain reaction (qPCR). RNAi (RNA interference) was used for knockdown in HNSCC cell lines, and the changes of malignant phenotype in the tumor cells after knockdown were examined by cell counting kit-8 (CCK-8), colony formation, flow cytometry, transwell invasion and migration assays. Bioinformatics analysis was performed to construct a -centered competing endogenous RNA (ceRNA) regulatory network, and GO (Gene Ontology) enrichment analysis was performed. Statistical analysis and graphing were performed using SPSS 25.0 software and GraphPad Prism 6 software. Mean levels in HNSCC tissues and TCGA database were higher than that in normal control tissues, but with no significantly statistical difference (0.522). expression levels were positively correlated with cervical lymph node metastasis and pathological grade in patients with HNSCC, with higher expression in male patients than in female patients (0.05). Transcriptome sequencing analysis showed that mean expression level of in LSCC tissues of 27 patients was significantly higher than that in the paired adjacent normal mucosa tissues (1.56, 0.036). expression was significantly upregulated in HNSCC cell lines FD-LSC-1, CAL-27 and Detroit562 (-values of -12.17, -23.26 and -388.57, respectively; all 0.001). Knockdown of by transfecting si--1 and si--2 inhibited HNSCC cell proliferation (-values of 8.95 and 4.84, 2.70 and 5.55, 2.02 and 3.70, respectively), colony formation (-values of 6.66 and 6.17, 7.38 and 11.65, 4.90 and 5.79, respectively), migration (-values of 8.21 and 7.19, 5.76 and 6.46, 6.28 and 9.92, respectively) and invasion abilities (-values of 9.29 and 10.25, 11.30 and 11.36, 8.02 and 8.66, respectively), but promoting apoptosis in cell lines FD-LSC-1 and CAL-27 (-values of -2.21 and -5.83, -3.05 and -5.25 respectively) (all -values<0.05). The -centered ceRNA network consists of 10 downregulated microRNA and 647 upregulated mRNA nodes. GO analysis results indicated that -regulated mRNAs were enriched in 22 biological processes, 32 molecular functions, and 12 cellular components. High level of is associated with the malignant progression of HNSCC. promotes the proliferation, migration, invasion, and antagonizes apoptosis of HNSCC cells, which serves as a potential molecular marker in HNSCC.