Xu Ruoqing, Dai Fei, Wu Honggang, Jiao Renjie, He Feng, Ma Jun
Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.
Institute of Genetics, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.
Heliyon. 2023 Feb 10;9(2):e13623. doi: 10.1016/j.heliyon.2023.e13623. eCollection 2023 Feb.
How patterns are formed to scale with tissue size remains an unresolved problem. Here we investigate embryonic patterns of gap gene expression along the anterior-posterior (AP) axis in . We use embryos that greatly differ in length and, importantly, possess distinct length-scaling characteristics of the Bicoid (Bcd) gradient. We systematically analyze the dynamic movements of gap gene expression boundaries in relation to both embryo length and Bcd input as a function of time. We document the process through which such dynamic movements drive both an emergence of a global scaling landscape and evolution of boundary-specific scaling characteristics. We show that, despite initial differences in pattern scaling characteristics that mimic those of Bcd in the anterior, such characteristics of final patterns converge. Our study thus partitions the contributions of Bcd input and regulatory dynamics inherent to the AP patterning network in shaping embryonic pattern's scaling characteristics.
模式如何随着组织大小而形成规模仍是一个未解决的问题。在这里，我们研究了胚胎中沿前后（AP）轴的间隙基因表达模式。我们使用长度差异很大且重要的是具有不同的Bicoid（Bcd）梯度长度缩放特征的胚胎。我们系统地分析了间隙基因表达边界相对于胚胎长度和作为时间函数的Bcd输入的动态运动。我们记录了这样的动态运动驱动全局缩放格局出现和边界特定缩放特征演变的过程。我们表明，尽管模式缩放特征的初始差异模仿了前部Bcd的差异，但最终模式的此类特征会趋同。因此，我们的研究划分了Bcd输入和AP模式化网络固有的调节动力学在塑造胚胎模式缩放特征方面的贡献。
