Martins de Sousa Karolinne, Linklater Denver P, Murdoch Billy J, Al Kobaisi Mohammad, Crawford Russell J, Judge Roy, Dashper Stuart, Sloan Alastair J, Losic Dusan, Ivanova Elena P
School of Science, STEM College, RMIT University, Melbourne, Victoria 3000, Australia.
Department of Biomedical Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia.
ACS Appl Bio Mater. 2023 Mar 20;6(3):1054-1070. doi: 10.1021/acsabm.2c00952. Epub 2023 Mar 7.
Despite recent advances in the development of orthopedic devices, implant-related failures that occur as a result of poor osseointegration and nosocomial infection are frequent. In this study, we developed a multiscale titanium (Ti) surface topography that promotes both osteogenic and mechano-bactericidal activity using a simple two-step fabrication approach. The response of MG-63 osteoblast-like cells and antibacterial activity toward and bacteria was compared for two distinct micronanoarchitectures of differing surface roughness created by acid etching, using either hydrochloric acid (HCl) or sulfuric acid (HSO), followed by hydrothermal treatment, henceforth referred to as either MN-HCl or MN-HSO. The MN-HCl surfaces were characterized by an average surface microroughness () of 0.8 ± 0.1 μm covered by blade-like nanosheets of 10 ± 2.1 nm thickness, whereas the MN-HSO surfaces exhibited a greater value of 5.8 ± 0.6 μm, with a network of nanosheets of 20 ± 2.6 nm thickness. Both micronanostructured surfaces promoted enhanced MG-63 attachment and differentiation; however, cell proliferation was only significantly increased on MN-HCl surfaces. In addition, the MN-HCl surface exhibited increased levels of bactericidal activity, with only 0.6% of the cells and approximately 5% cells remaining viable after 24 h when compared to control surfaces. Thus, we propose the modulation of surface roughness and architecture on the micro- and nanoscale to achieve efficient manipulation of osteogenic cell response combined with mechanical antibacterial activity. The outcomes of this study provide significant insight into the further development of advanced multifunctional orthopedic implant surfaces.
尽管近年来骨科器械的发展取得了进展，但由于骨整合不良和医院感染导致的植入物相关失败仍很常见。在本研究中，我们开发了一种多尺度钛（Ti）表面形貌，采用简单的两步制造方法促进成骨和机械杀菌活性。使用盐酸（HCl）或硫酸（H₂SO₄）进行酸蚀刻，然后进行水热处理，从而形成两种具有不同表面粗糙度的独特微米纳米结构，即MN-HCl和MN-HSO，比较了MG-63成骨样细胞对这两种结构的反应以及对金黄色葡萄球菌和大肠杆菌的抗菌活性。MN-HCl表面的特征是平均表面微观粗糙度（Rₐ）为0.8±0.1μm，覆盖着厚度为10±2.1nm的叶片状纳米片，而MN-HSO表面的Rₐ值更大，为5.8±0.6μm，具有厚度为20±2.6nm的纳米片网络。两种微米纳米结构表面均促进了MG-63细胞的附着和分化增强；然而，仅MN-HCl表面的细胞增殖显著增加。此外，MN-HCl表面的杀菌活性水平提高，与对照表面相比，24小时后金黄色葡萄球菌细胞仅0.6%存活，大肠杆菌细胞约5%存活。因此，我们提出在微米和纳米尺度上调节表面粗糙度和结构，以实现对成骨细胞反应的有效调控并兼具机械抗菌活性。本研究结果为先进多功能骨科植入物表面的进一步发展提供了重要见解。
