Cancer Target and Experimental Therapeutics, Institute for Advanced Biosciences, INSERM U1209, UMR CNRS 5309, Grenoble Alpes University, Site Santé, Allée des Alpes, 38700 La Tronche, France.
School of Science, RMIT University, Melbourne, VIC 3000, Australia.
ACS Appl Mater Interfaces. 2020 Oct 28;12(43):48272-48283. doi: 10.1021/acsami.0c11502. Epub 2020 Oct 15.
Despite advances in the development of bone substitutes and strict aseptic procedures, the majority of failures in bone grafting surgery are related to nosocomial infections. Development of biomaterials combining both osteogenic and antibiotic activity is, therefore, a crucial public health issue. Herein, two types of intrinsically bactericidal titanium supports were fabricated by using commercially scalable techniques: plasma etching or hydrothermal treatment, which display two separate mechanisms of mechano-bactericidal action. Hydrothermal etching produces a randomly nanostructured surface with sharp nanosheet protrusions killing bacteria via cutting of the cell membrane, whereas plasma etching of titanium produces a microscale two-tier hierarchical topography that both reduce bacterial attachment and rupture those bacteria that encounter the surface. The adhesion, growth, and proliferation of human adipose-derived stem cells (hASCs) on the two mechano-bactericidal topographies were assessed. Both types of supports allowed the growth and proliferation of the hASCs in the same manner and cells retained their stemness and osteogenic potential. Furthermore, these supports induced osteogenic differentiation of hASCs without the need of differentiation factors, demonstrating their osteoinductive properties. This study proves that these innovative mechano-bactericidal titanium surfaces with both regenerative and bactericidal properties are a promising solution to improve the success rate of reconstructive surgery.
尽管在骨替代物的开发和严格的无菌操作方面取得了进展，但大多数骨移植手术的失败都与医院获得性感染有关。因此，开发兼具成骨和抗生素活性的生物材料是一个至关重要的公共卫生问题。本文采用商业上可扩展的技术，即等离子体蚀刻或水热处理，制备了两种具有内在杀菌能力的钛支架：水热处理产生具有锋利纳米片突起的随机纳米结构表面，通过切割细胞膜杀死细菌，而钛的等离子体蚀刻则产生微尺度的两级分层形貌，既能减少细菌附着，又能破坏遇到表面的细菌。评估了两种机械杀菌拓扑结构对人脂肪来源干细胞（hASC）的黏附、生长和增殖的影响。这两种支架都以相同的方式允许 hASC 的生长和增殖，并且细胞保持其干性和成骨潜能。此外，这些支架无需分化因子即可诱导 hASC 成骨分化，显示出其成骨诱导特性。这项研究证明，这些具有再生和杀菌性能的创新机械杀菌钛表面是提高重建手术成功率的有前途的解决方案。
