MoS/PDA@Cu composite as a peroxidase-mimicking enzyme with high-effect antibacterial and anticancer activity.

Since nanozymes were proposed, their applications have become more and more extensive. As a research hotspot in recent years, MoS also shows many enzyme-like properties. However, as a novel peroxidase, MoS has the disadvantage of a low maximum reaction rate. In this study, the MoS/PDA@Cu nanozyme was synthesized by a wet chemical method. The modification of PDA on the surface of MoS achieved the uniform growth of small-sized Cu Nps. The obtained MoS/PDA@Cu nanozyme displayed excellent peroxidase-like activity and antibacterial properties. The minimum inhibitory concentration (MIC) of the MoS/PDA@Cu nanozyme against reached 25 μg mL. Furthermore, it showed a more pronounced inhibitory effect on bacterial growth with the addition of HO. The maximum reaction rate () of the MoS/PDA@Cu nanozyme is 29.33 × 10 M s, which is significantly higher as compared to that of HRP. It also exhibited excellent biocompatibility, hemocompatibility and potential anticancer properties. When the concentration of the nanozyme was 160 μg mL, the viabilities of 4T1 cells and Hep G2 cells were 45.07% and 32.35%, respectively. This work indicates that surface regulation and electronic transmission control are good strategies for improving peroxidase-like activity.