Australian Infectious Disease Research Centre, School of Biological Sciences, The University of Queensland, Brisbane, QLD, 4072, Australia.
Australian Infectious Disease Research Centre, School of Biological Sciences, The University of Queensland, Brisbane, QLD, 4072, Australia.
Virology. 2023 Apr;581:48-55. doi: 10.1016/j.virol.2023.02.017. Epub 2023 Mar 3.
Wolbachia pipientis is known to block replication of positive sense RNA viruses. Previously, we created an Aedes aegypti Aag2 cell line (Aag2.wAlbB) transinfected with the wAlbB strain of Wolbachia and a matching tetracycline-cured Aag2.tet cell line. While dengue virus (DENV) was blocked in Aag2.wAlbB cells, we found significant inhibition of DENV in Aag2.tet cells. RNA-Seq analysis of the cells confirmed removal of Wolbachia and lack of expression of Wolbachia genes that could have been due to lateral gene transfer in Aag2.tet cells. However, we noticed a substantial increase in the abundance of phasi charoen-like virus (PCLV) in Aag2.tet cells. When RNAi was used to reduce the PCLV levels, DENV replication was significantly increased. Further, we found significant changes in the expression of antiviral and proviral genes in Aag2.tet cells. Overall, the results reveal an antagonistic interaction between DENV and PCLV and how PCLV-induced changes could contribute to DENV inhibition.
已知沃尔巴克氏体（Wolbachia pipientis）可阻止正链 RNA 病毒的复制。此前，我们创建了一种转染了沃尔巴克氏体 wAlbB 株和相应的四环素治愈的 Aag2.tet 细胞系的埃及伊蚊 Aag2 细胞系（Aag2.wAlbB）。虽然登革热病毒（DENV）在 Aag2.wAlbB 细胞中被阻断，但我们发现 DENV 在 Aag2.tet 细胞中受到显著抑制。细胞的 RNA-Seq 分析证实沃尔巴克氏体已被去除，并且不存在可能由于 Aag2.tet 细胞中的横向基因转移而产生的沃尔巴克氏体基因表达。然而，我们注意到 Aag2.tet 细胞中 phasi charoen-like virus（PCLV）的丰度大量增加。当使用 RNAi 降低 PCLV 水平时，DENV 复制显著增加。此外，我们发现 Aag2.tet 细胞中抗病毒和前病毒基因的表达发生了显著变化。总体而言，结果揭示了 DENV 和 PCLV 之间的拮抗相互作用，以及 PCLV 诱导的变化如何有助于 DENV 抑制。
