Department of Gastroenterology, Southern University of Science and Technology Hospital, Shenzhen, China.
Department of Pathology, Southern University of Science and Technology Hospital, Shenzhen, China.
Helicobacter. 2023 Jun;28(3):e12965. doi: 10.1111/hel.12965. Epub 2023 Mar 8.
Most patients with Helicobacter pylori (H. pylori) infection have no clinical symptoms, numerous studies reported the gastric microbiome in H. pylori-infected patients, but asymptomatic patients have not been distinguished. How the microbiome and function changes in asymptomatic patients with H. pylori infection remains poorly understood.
A total of 29 patients were divided into H. pylori-infected asymptomatic group (10 patients), H. pylori-infected symptomatic group (11 patients) and H. pylori-uninfected group (8 patients). Gastric mucosa specimens were taken for histopathological examination, special staining, and 16 S rDNA sequencing. High-throughput results were evaluated by community composition analysis, indicator species analysis, alpha diversity analysis, beta diversity analysis, and function prediction.
The gastric microbiota composition at phylum and genus level of H. pylori-infected asymptomatic patients were similar with H. pylori-infected symptomatic group, but different from H. pylori-uninfected patients. The diversity and richness of gastric microbial community declined significantly in H. pylori-infected asymptomatic group comparing with H. pylori-uninfected group. Sphingomonas may be an indicator between symptomatic and asymptomatic patients with H. pylori infection, the AUC value of Sphingomonas is 0.79. Interactions between species increased and altered notably after H. pylori infection. More genera were affected by Helicobacter in H. pylori-infected asymptomatic patients. The function condition changed significantly in asymptomatic patients with H. pylori infection, there was no difference comparing with symptomatic ones. Amino acid metabolism and lipid metabolism strengthened but carbohydrate metabolism remained constant after H. pylori infection. The metabolism of fatty acid and bile acid was disturbed after infection with H. pylori.
The gastric microbiota composition and function mode changed significantly after H. pylori infection regardless of the presence of clinical symptoms, there was no difference between H. pylori-infected asymptomatic and symptomatic patients. The difference in gastric microbiota composition and interactions between species might be responsible for presence of digestive symptoms.
大多数幽门螺杆菌（H. pylori）感染患者没有临床症状，许多研究报道了 H. pylori 感染患者的胃微生物组，但未区分无症状患者。无症状 H. pylori 感染患者的微生物组和功能如何变化尚不清楚。
共纳入 29 例患者，分为 H. pylori 感染无症状组（10 例）、H. pylori 感染有症状组（11 例）和 H. pylori 未感染组（8 例）。取胃黏膜标本进行组织病理学检查、特殊染色和 16S rDNA 测序。通过群落组成分析、指示物种分析、α多样性分析、β多样性分析和功能预测评估高通量结果。
H. pylori 感染无症状患者的胃微生物群组成在门和属水平上与 H. pylori 感染有症状组相似，但与 H. pylori 未感染患者不同。与 H. pylori 未感染组相比，H. pylori 感染无症状组胃微生物群落的多样性和丰富度显著下降。变形菌门的 Sphingomonas 可能是 H. pylori 感染有症状和无症状患者之间的指示菌，Sphingomonas 的 AUC 值为 0.79。H. pylori 感染后，种间相互作用增加且显著改变。更多的菌属受 H. pylori 感染的影响。H. pylori 感染无症状患者的功能状况发生显著变化，但与有症状患者无差异。H. pylori 感染后，氨基酸代谢和脂质代谢增强，但碳水化合物代谢保持不变。H. pylori 感染后，脂肪酸和胆汁酸代谢紊乱。
无论有无临床症状，H. pylori 感染后胃微生物群组成和功能模式均发生显著变化，H. pylori 感染无症状和有症状患者之间无差异。胃微生物群组成和种间相互作用的差异可能是引起消化道症状的原因。
