Jiang Zhengjie, Zhao Mengyu, Qin Hongzhen, Li Sicheng, Yang Xiping
State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Key Laboratory of Sugarcane Biology, Guangxi University, Nanning, China.
National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, China.
Front Plant Sci. 2023 Feb 20;14:1091567. doi: 10.3389/fpls.2023.1091567. eCollection 2023.
During plant evolution, nucleotide-binding sites (NBS) and leucine-rich repeat (LRR) genes have made significant contributions to plant disease resistance. With many high-quality plant genomes sequenced, identification and comprehensive analyses of NBS-LRR genes at whole genome level are of great importance to understand and utilize them.
In this study, we identified the NBS-LRR genes of 23 representative species at whole genome level, and researches on NBS-LRR genes of four monocotyledonous grass species, Saccharum spontaneum, Saccharum officinarum, Sorghum bicolor and Miscanthus sinensis, were focused.
We found that whole genome duplication, gene expansion, and allele loss could be factors affecting the number of NBS-LRR genes in the species, and whole genome duplication is likely to be the main cause of the number of NBS-LRR genes in sugarcane. Meanwhile, we also found a progressive trend of positive selection on NBS-LRR genes. These studies further elucidated the evolutionary pattern of NBS-LRR genes in plants. Transcriptome data from multiple sugarcane diseases revealed that more differentially expressed NBS-LRR genes were derived from S. spontaneum than from S. officinarum in modern sugarcane cultivars, and the proportion was significantly higher than the expected. This finding reveals that S. spontaneum has a greater contribution to disease resistance for modern sugarcane cultivars. In addition, we observed allelespecific expression of seven NBS-LRR genes under leaf scald, and 125 NBS-LRR genes responding to multiple diseases were identified. Finally, we built a plant NBS-LRR gene database to facilitate subsequent analysis and use of NBSLRR genes obtained here. In conclusion, this study complemented and completed the research of plant NBS-LRR genes, and discussed how NBS-LRR genes responding to sugarcane diseases, which provided a guide and genetic resources for further research and utilization of NBS-LRR genes.
在植物进化过程中，核苷酸结合位点（NBS）和富含亮氨酸重复序列（LRR）基因对植物抗病性做出了重要贡献。随着许多高质量植物基因组被测序，在全基因组水平上鉴定和全面分析NBS-LRR基因对于理解和利用它们至关重要。
在本研究中，我们在全基因组水平上鉴定了23个代表性物种的NBS-LRR基因，并重点研究了四个单子叶禾本科物种，即野生甘蔗、甘蔗、高粱和芒草的NBS-LRR基因。
我们发现全基因组复制、基因扩增和等位基因丢失可能是影响物种中NBS-LRR基因数量的因素，并且全基因组复制可能是甘蔗中NBS-LRR基因数量的主要原因。同时，我们还发现了NBS-LRR基因上正向选择的渐进趋势。这些研究进一步阐明了植物中NBS-LRR基因的进化模式。来自多种甘蔗病害的转录组数据显示，在现代甘蔗品种中，来自野生甘蔗的差异表达NBS-LRR基因比来自甘蔗的更多，且该比例显著高于预期。这一发现表明野生甘蔗对现代甘蔗品种的抗病性贡献更大。此外，我们观察到七个NBS-LRR基因在叶瘟病下的等位基因特异性表达，并鉴定出125个对多种病害有响应的NBS-LRR基因。最后，我们构建了一个植物NBS-LRR基因数据库，以方便后续对这里获得的NBS-LRR基因进行分析和使用。总之，本研究补充并完成了植物NBS-LRR基因的研究，并探讨了NBS-LRR基因对甘蔗病害的响应，为进一步研究和利用NBS-LRR基因提供了指导和遗传资源。
