Lin Haidong, Jiang Zhengjie, He Tuan, Li Guomeng, Zhao Mengyu, Su Liangyinan, Zhao Jihan, Zou Chengwu, Yang Xiping
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530005, China.
Guangxi Key Laboratory of Sugarcane Biology & National Demonstration Center for Experimental Plant Science Education, Guangxi University, Nanning 530005, China.
Plants (Basel). 2024 Dec 14;13(24):3497. doi: 10.3390/plants13243497.
Sugarcane Pokkah Boeng (PB), a fungal disease caused by spp., poses a significant threat to sugar industries globally. Breeding sugarcane varieties resistant to PB has become a priority, and the mining of PB resistance genes and the development of molecular markers provide a solid foundation for this purpose. This work comprehensively analyzes the genetic components of sugarcane's resistance to PB using transcriptome sequencing. A segregating population was created by crossing the susceptible parent ROC25 with the resistant parent Yunzhe89-7, which is a traditional cultivar known for its PB resistance. Transcriptome analysis uncovered many differentially expressed genes (DEGs) associated with PB resistance. Utilizing weighted gene co-expression network analysis (WGCNA), we identified gene modules closely related to disease phenotypes. We annotated their functions with bioinformatics tools, particularly focusing on genes enriched in the plant immune response's MAPK signaling pathway and ABC transporter synthesis pathways. In addition, by integrating whole-genome resequencing data of parental lines and transcriptome data of progeny, we identified a series of putative molecular markers that potentially effectively differentiate between highly resistant and susceptible materials. Our study provides crucial genetic resources and molecular methodologies that are essential for the advancement of sugarcane varieties with improved resistance to PB. These innovations are expected to accelerate the breeding process greatly.
甘蔗梢腐病（PB）是由 spp.引起的一种真菌病害，对全球甘蔗产业构成重大威胁。培育抗PB的甘蔗品种已成为当务之急，挖掘PB抗性基因并开发分子标记为此提供了坚实基础。本研究利用转录组测序全面分析了甘蔗对PB抗性的遗传成分。通过将感病亲本ROC25与抗性亲本云蔗89-7杂交构建了一个分离群体，云蔗89-7是一个以抗PB而闻名的传统品种。转录组分析发现了许多与PB抗性相关的差异表达基因（DEG）。利用加权基因共表达网络分析（WGCNA），我们鉴定出了与疾病表型密切相关的基因模块。我们用生物信息学工具注释了它们的功能，特别关注富集在植物免疫反应的MAPK信号通路和ABC转运蛋白合成通路中的基因。此外，通过整合亲本的全基因组重测序数据和子代的转录组数据，我们鉴定出了一系列可能有效区分高抗和感病材料的假定分子标记。我们的研究提供了关键的遗传资源和分子方法，这些对于培育抗PB能力更强的甘蔗品种至关重要。预计这些创新将大大加快育种进程。
