Thepbandit Wannaporn, Srisuwan Anake, Siriwong Supatcharee, Nawong Siriwan, Athinuwat Dusit
Faculty of Science and Technology, Thammasat University, Pathumtani, Thailand.
Faculty of Science and Technology, Nakhon Ratchasima Rajabhat University, Nakhon Ratchasima, Thailand.
Front Plant Sci. 2023 Feb 20;14:1103487. doi: 10.3389/fpls.2023.1103487. eCollection 2023.
Beneficial microorganisms are an important strategy for sustainable plant production processes such as stimulate root exudation, stress tolerance, and yield improvement. This study investigated various microorganisms isolated from the rhizosphere of L. in order to inhibit cause of rice blast, by direct and indirect mode of action. The results indicated that strain TU-Orga21 significantly reduced mycelium growth and deformed the hyphal structures. The effects of biosurfactant TU-Orga21 was studied against spore development. The dose of ≥5% / biosurfactant significantly inhibited the germ tubes and appressoria formation. The biosurfactants were evaluated as surfactin and iturin A by Matrix-assisted laser desorption ionization dual time-of-flight tandem mass spectrometry. Under greenhouse conditions, priming the biosurfactant three times before infection significantly accumulated endogenous salicylic acid, phenolic compounds, and hydrogen peroxide (HO) during the infection process of . The SR-FT-IR spectral changes from the mesophyll revealed higher integral area groups of lipids, pectins, and proteins amide I and amide II in the elicitation sample. Furthermore, scanning electron microscope revealed appressorium and hyphal enlargement in un-elicitation leaves whereas appressorium formation and hyphal invasion were not found in biosurfactant-elicitation at 24 h post inoculation. The biosurfactant treatment significantly mitigated rice blast disease severity. Therefore, can be a promising novel biocontrol agent which contains the preformed active metabolites for a rapid control of rice blast by a direct action against pathogen and by boosting plant immunity.
有益微生物是可持续植物生产过程中的一项重要策略，例如刺激根系分泌、提高抗逆性和增加产量。本研究调查了从L.根际分离出的各种微生物，以通过直接和间接作用方式抑制稻瘟病的病因。结果表明，菌株TU-Orga21显著降低了菌丝体生长并使菌丝结构变形。研究了生物表面活性剂TU-Orga21对孢子发育的影响。生物表面活性剂剂量≥5%时显著抑制了芽管和附着胞的形成。通过基质辅助激光解吸电离双飞行时间串联质谱法将生物表面活性剂鉴定为表面活性素和伊枯草菌素A。在温室条件下，在感染前对生物表面活性剂进行三次引发处理，在稻瘟病菌感染过程中显著积累了内源性水杨酸、酚类化合物和过氧化氢（HO）。叶肉的SR-FT-IR光谱变化显示，诱导样品中脂质、果胶以及蛋白质酰胺I和酰胺II的积分面积组更高。此外，扫描电子显微镜显示，未诱导的叶片中有附着胞和菌丝膨大，而在接种后24小时的生物表面活性剂诱导处理中未发现附着胞形成和菌丝入侵。生物表面活性剂处理显著减轻了稻瘟病的严重程度。因此，它可能是一种有前景的新型生物防治剂，其含有预先形成的活性代谢产物，可通过直接作用于病原体和增强植物免疫力来快速控制稻瘟病。
