Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand.
Center of Excellence in Natural Products for Ageing and Chronic Diseases, Chulalongkorn University, Bangkok, 10330, Thailand.
Chem Biodivers. 2023 Apr;20(4):e202201096. doi: 10.1002/cbdv.202201096. Epub 2023 Apr 19.
The objective of this study was to evaluate the antioxidant, anti-skin-aging, anti-inflammatory, and anti-acetylcholinesterase activities of the hexane (n-hex), AcOEt, BuOH, MeOH, and aqueous extracts from R. oligophlebia roots. The total phenolic and flavonoid contents (TPC and TFC) were determined using Folin-Ciocalteu and AlCl colorimetric assays. The antioxidant capacity was examined by reducing power (RP), ferric reducing antioxidant power (FRAP), ABTS⋅ , and DPPH⋅ radical cation assays. All extracts potentially exhibited antioxidant activity with IC values ranging from 2.93 to 5.73 μg/mL for ABTS⋅ and from 5.69 to 7.65 μg/mL for DPPH⋅ except the n-hex extract. The BuOH, MeOH, and aqueous extract possess promising anti-skin-aging activities, as observed by an attenuation of UV-A toxicity on human keratinocytes. We proposed that these anti-skin-aging properties are possibly due to direct scavenging activity against reactive oxygen species and upregulate cellular antioxidant machinery. Moreover, we found that the antioxidant capacity was well correlated with anti-inflammatory capacity against nitric oxide (NO) production in terms of the n-hex, AcOEt, and BuOH extracts with IC values from 23.21 to 47.1 μg/mL. In contrast, these activities were found to be poorly correlated with AchE activity. To the best of our knowledge, this is the first report of the antioxidant, anti-skin-aging, anti-inflammatory, and anti-acetylcholinesterase activities of the extracts of R. oligophlebia roots. These findings indicated that this species could be a potential source of natural antioxidant, anti-aging, and anti-inflammatory agents. Consequently, it may be suggested as a medicinal plant that prevents diseases related to oxidative stress and inflammatory responses.
本研究旨在评估 R. oligophlebia 根的正己烷（n-hex）、乙酸乙酯（AcOEt）、正丁醇（BuOH）、甲醇（MeOH）和水提物的抗氧化、抗皮肤老化、抗炎和抗乙酰胆碱酯酶活性。采用 Folin-Ciocalteu 和 AlCl 比色法测定总酚和总黄酮含量（TPC 和 TFC）。通过还原能力（RP）、铁还原抗氧化能力（FRAP）、ABTS·和 DPPH·自由基阳离子测定法来检测抗氧化能力。所有提取物均表现出潜在的抗氧化活性，ABTS·的 IC 值范围为 2.93 至 5.73μg/mL，DPPH·的 IC 值范围为 5.69 至 7.65μg/mL，除了 n-hex 提取物。BuOH、MeOH 和水提物具有显著的抗皮肤老化活性，这可通过减轻人角质形成细胞对 UV-A 毒性的作用来观察到。我们提出，这些抗皮肤老化特性可能是由于对活性氧的直接清除活性和上调细胞抗氧化机制所致。此外，我们发现抗氧化能力与抗一氧化氮（NO）生成的抗炎能力密切相关，n-hex、AcOEt 和 BuOH 提取物的 IC 值范围为 23.21 至 47.1μg/mL。相比之下，这些活性与 AchE 活性相关性较差。据我们所知，这是首次报道 R. oligophlebia 根提取物的抗氧化、抗皮肤老化、抗炎和抗乙酰胆碱酯酶活性。这些发现表明，该物种可能是天然抗氧化剂、抗衰老和抗炎剂的潜在来源。因此，它可能被推荐为一种预防与氧化应激和炎症反应相关疾病的药用植物。
