基于微型质谱仪的即时检测法测定血液中的磷脂酰乙醇。
Miniature mass spectrometer-based point-of-care assay for measuring phosphatidylethanol in blood.
机构信息
Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado Denver, Aurora, CO 80045, USA.
出版信息
Analyst. 2023 Mar 27;148(7):1430-1436. doi: 10.1039/d3an00098b.
Abstract
We demonstrate proof-of-concept for point-of-care assessment of long-term alcohol consumption by measuring phosphatidylethanol in blood/dried blood spots with nano-electrospray ionization and MS/MS using a miniature mass spectrometer. 'Abstinence', 'moderate', and 'chronic' consumption could be distinguished rapidly for both sample types, and quantitative performance was obtained with blood (LoQ-100 ng mL).
摘要
我们通过使用微型质谱仪，以纳喷雾离子化和 MS/MS 的方式，在血液/干血斑中测量磷脂酰乙醇，从而证明了即时检测长期酒精摄入的概念验证。对于这两种样本类型，都可以快速区分“禁欲”、“适度”和“慢性”摄入，并且血液中可获得定量性能（LoQ-100ng/mL）。
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