使用微型质谱仪快速鉴别细菌。

Rapid discrimination of bacteria using a miniature mass spectrometer.

作者信息

Pulliam Christopher J, Wei Pu, Snyder Dalton T, Wang Xiao, Ouyang Zheng, Pielak Rafal M, Graham Cooks R

机构信息

Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.

Department of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA.

出版信息

Analyst. 2016 Mar 7;141(5):1633-6. doi: 10.1039/c5an02575c.

DOI:10.1039/c5an02575c

PMID:26844973

Abstract

Bacteria colonies were analyzed using paper spray ionization coupled with a portable mass spectrometer. The spectra were averaged and processed using multivariate analysis to discriminate between different species of bacteria based on their unique phospholipid profiles. Full scan mass spectra and product ion MS/MS data were compared to those recorded using a benchtop linear ion trap mass spectrometer.

摘要

使用纸喷雾电离结合便携式质谱仪对细菌菌落进行分析。对光谱进行平均，并使用多变量分析进行处理，以根据不同细菌物种独特的磷脂谱来区分它们。将全扫描质谱和产物离子MS/MS数据与使用台式线性离子阱质谱仪记录的数据进行比较。