含 8-三氟甲基-2'-脱氧鸟苷的寡核苷酸作为 Z-DNA 探针。

Oligonucleotide Containing 8-Trifluoromethyl-2'-Deoxyguanosine as a Z-DNA Probe.

机构信息

Division of Chemistry, Department of Medical Sciences, Faculty of Medicine, University of Miyazaki, Kiyotake, Miyazaki, Japan.

Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

出版信息

Methods Mol Biol. 2023;2651:115-130. doi: 10.1007/978-1-0716-3084-6_8.

DOI:10.1007/978-1-0716-3084-6_8

PMID:36892763

Abstract

Z-DNA structure is a noncanonical left-handed alternative form of DNA, which has been suggested to be biologically important and is related to several genetic diseases and cancer. Therefore, investigation of Z-DNA structure associated with biological events is of great importance to understanding the functions of these molecules. Here, we described the development of a trifluoromethyl labeled deoxyguanosine derivative and employed it as a F NMR probe to study Z-form DNA structure in vitro and in living cells.

摘要

Z-DNA 结构是 DNA 的一种非canonical 左手替代形式，它被认为具有重要的生物学意义，并与几种遗传疾病和癌症有关。因此，研究与生物事件相关的 Z-DNA 结构对于理解这些分子的功能非常重要。在这里，我们描述了一种三氟甲基标记的脱氧鸟苷衍生物的开发，并将其用作 F NMR 探针，用于研究体外和活细胞中的 Z 型 DNA 结构。