Lacotte Virginie, Dell'Aglio Elisa, Peignier Sergio, Benzaoui Fadéla, Heddi Abdelaziz, Rebollo Rita, Da Silva Pedro
Univ Lyon, INSA Lyon, INRAE, BF2I, UMR 203, 69621 Villeurbanne, France.
Heliyon. 2023 Feb 23;9(3):e13962. doi: 10.1016/j.heliyon.2023.e13962. eCollection 2023 Mar.
Cereal-feeding beetles are a major risk for cereal crop maintenance. Cereal weevils such as have symbiotic intracellular bacteria that provide essential aromatic amino acid to the host for the biosynthesis of their cuticle building blocks. Their cuticle is an important protective barrier against biotic and abiotic stresses, providing high resistance from insecticides. Quantitative optical methods specialized in insect cuticle analysis exist, but their scope of use and the repeatability of the results remain limited. Here, we investigated the potential of Hyperspectral Imaging (HSI) as a standardized cuticle analysis tool. Based on HSI, we acquired time series of average reflectance profiles from 400 to 1000 nm from symbiotic (with bacteria) and aposymbiotic (without bacteria) cereal weevils exposed to different nutritional stresses. We assessed the phenotypic changes of weevils under different diets throughout their development and demonstrated the agreement of the results between the HSI method and the classically used Red-Green-Blue analysis. Then, we compared the use of both technologies in laboratory conditions and highlighted the assets of HSI to develop a simple, automated, and standardized analysis tool. This is the first study showing the reliability and feasibility of HSI for a standardized analysis of insect cuticle changes.
以谷物为食的甲虫是谷物作物养护的一大风险。诸如谷物象鼻虫之类的昆虫具有共生的细胞内细菌，这些细菌为宿主提供必需的芳香族氨基酸，用于其表皮构建模块的生物合成。它们的表皮是抵御生物和非生物胁迫的重要保护屏障，对杀虫剂具有高度抗性。虽然存在专门用于昆虫表皮分析的定量光学方法，但其使用范围和结果的可重复性仍然有限。在此，我们研究了高光谱成像（HSI）作为一种标准化表皮分析工具的潜力。基于高光谱成像，我们获取了来自共生（有细菌）和非共生（无细菌）的谷物象鼻虫在不同营养胁迫下400至1000纳米的平均反射率曲线的时间序列。我们评估了象鼻虫在不同饮食条件下整个发育过程中的表型变化，并证明了高光谱成像方法与传统使用的红-绿-蓝分析结果的一致性。然后，我们比较了这两种技术在实验室条件下的使用情况，并强调了高光谱成像在开发一种简单、自动化和标准化分析工具方面的优势。这是第一项展示高光谱成像用于昆虫表皮变化标准化分析的可靠性和可行性的研究。
