DE Carvalho Carlos Dellavechia, Filho Carlos Augusto Kalva, DA Rocha Alisson Luiz, Silva Adelino Sanchez Ramos, Papoti Marcelo
Ribeirão Preto Medical School, University of São Paulo, SP, BRAZIL.
Physical Education, School of Sciences, São Paulo State University, SP, BRAZIL.
Int J Exerc Sci. 2022 Feb 1;15(2):289-299. doi: 10.70252/DAEM9945. eCollection 2022.
The aim of this study is to verify the influence of the intensity on muscle and hepatic glycogen depletion and recovery kinetics of Wistar rats, submitted to three acute training sessions with equalized loads. 81 male Wistar rats performed an incremental test to determine maximal running speed (MRS) and divided into 4 groups: baseline group (Control; 9); low intensity training session (G; 24; 48 minutes at 50% of MRS); moderate intensity group (G; 24; 32 minutes at 75% of MRS) and high intensity group (G; 24; 5x5 minutes and 20 seconds at 90% of MRS). Immediately after the sessions and after 6, 12 and 24 hours, 6 animals from each subgroup were euthanized for glycogen quantification in soleus and EDL muscles and liver. A Two-Way ANOVA and the Fisher's Post-hoc test was used ( 0.05). Glycogen supercompensation occurred between 6 and 12 hours after exercise in muscle tissue and 24 after exercise in the liver. The muscle and hepatic glycogen depletion and recovery kinetics are not modulated by exercise intensity since the load was equalized, but effects were distinct in different tissues. Hepatic glycogenolysis and muscle glycogen synthesis processes seem to run in parallel.
本研究的目的是验证强度对Wistar大鼠肌肉和肝糖原消耗及恢复动力学的影响，这些大鼠接受了三次负荷相等的急性训练。81只雄性Wistar大鼠进行递增测试以确定最大跑步速度（MRS），并分为4组：基线组（对照组；9只）；低强度训练组（G组；24只；以MRS的50%跑48分钟）；中等强度组（G组；24只；以MRS的75%跑32分钟）和高强度组（G组；24只；以MRS的90%跑5×5分钟加20秒）。训练结束后以及6、12和24小时后，每个亚组处死6只动物，用于检测比目鱼肌、趾长伸肌和肝脏中的糖原含量。采用双向方差分析和Fisher事后检验（P<0.05）。肌肉组织中糖原超量补偿发生在运动后6至12小时，肝脏中发生在运动后24小时。由于负荷相等，运动强度未调节肌肉和肝糖原的消耗及恢复动力学，但不同组织中的效应不同。肝糖原分解和肌肉糖原合成过程似乎并行进行。
