Frerker Nadine, Karlsen Tommy A, Stensland Maria, Nyman Tuula A, Rayner Simon, Brinchmann Jan E
Department of Immunology, Oslo University Hospital, Oslo, Norway.
Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway.
Front Bioeng Biotechnol. 2023 Feb 21;11:1116513. doi: 10.3389/fbioe.2023.1116513. eCollection 2023.
Focal lesions of articular cartilage give rise to pain and reduced joint function and may, if left untreated, lead to osteoarthritis. Implantation of generated, scaffold-free autologous cartilage discs may represent the best treatment option. Here we compare articular chondrocytes (ACs) and bone marrow-derived mesenchymal stromal cells (MSCs) for their ability to make scaffold-free cartilage discs. Articular chondrocytes produced more extracellular matrix per seeded cell than mesenchymal stromal cells. Quantitative proteomics analysis showed that articular chondrocyte discs contained more articular cartilage proteins, while mesenchymal stromal cell discs had more proteins associated with cartilage hypertrophy and bone formation. Sequencing analysis revealed more microRNAs associated with normal cartilage in articular chondrocyte discs, and large-scale target predictions, performed for the first time for chondrogenesis, suggested that differential expression of microRNAs in the two disc types were important mechanisms behind differential synthesis of proteins. We conclude that articular chondrocytes should be preferred over mesenchymal stromal cells for tissue engineering of articular cartilage.
关节软骨的局灶性病变会引发疼痛并导致关节功能下降，若不加以治疗，可能会发展为骨关节炎。植入生成的、无支架的自体软骨盘可能是最佳治疗选择。在此，我们比较关节软骨细胞（ACs）和骨髓间充质基质细胞（MSCs）生成无支架软骨盘的能力。每个接种细胞，关节软骨细胞产生的细胞外基质比间充质基质细胞更多。定量蛋白质组学分析表明，关节软骨细胞盘含有更多的关节软骨蛋白，而间充质基质细胞盘含有更多与软骨肥大和骨形成相关的蛋白。测序分析显示，关节软骨细胞盘中有更多与正常软骨相关的微小RNA，首次针对软骨形成进行的大规模靶标预测表明，两种盘类型中微小RNA的差异表达是蛋白质差异合成背后的重要机制。我们得出结论，在关节软骨组织工程中，关节软骨细胞应优于间充质基质细胞。
