Darjanki Claudia Michelle, Prahasanti Chiquita, Fitria A Eka, Kusumawardani Banun, Wijaksana I Komang Evan, Aljunaid Mohammed
Residence in Periodontic Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Department of Periodontology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
J Oral Biol Craniofac Res. 2023 Mar-Apr;13(2):277-282. doi: 10.1016/j.jobcr.2023.02.007. Epub 2023 Feb 24.
To observe the expression of Runt-Related Transcription Factors 2 (RUNX2) and Alkaline Phosphatase (ALP) markers in osteoblast cell cultures exposed to Polymethylmethacrylate (PMMA) combined with hydroxyapatite (HAp) material to improve osteointegration of bone implants.
Sample of PMMA and HAp materials with a mixture of PMMA with HAp made from limestone as natural source which processed through Balai Besar Keramik (HApBBK) in the first group and a mixture of PMMA with HAp made from bovine bone which processed through Good Manufacturing Practice (HApGMP) in the second group. Twenty-four fetal rat calvarie osteoblast cell cultures were randomly divided into 6 groups: 7- and 14-day control group, 7 and 14 days PMMA-HApGMP group, 7 and 14 days PMMA-HApBBK group. The expression of RUNX2 and ALP was seen by immunocytochemical examination.
The one-way ANOVA with a significance value of 0.000 (p < 0.05). There was an increase in RUNX2 and ALP expressions on both PMMA-HApBBK and PMMA-HApGMP groups on days 7 and 14 in osteoblast cell cultures.
The PMMA-HApBBK and PMMA-HApGMP showed an increase in the RUNX2 and ALP expression in osteoblast cell cultures which indicates a potential increase of osseointegration of bone implants.
观察暴露于聚甲基丙烯酸甲酯（PMMA）与羟基磷灰石（HAp）复合材料的成骨细胞培养物中Runt相关转录因子2（RUNX2）和碱性磷酸酶（ALP）标志物的表达，以改善骨植入物的骨整合。
第一组为PMMA和HAp材料样本，其中PMMA与以石灰石为天然来源并经Balai Besar Keramik处理的HAp（HApBBK）混合；第二组为PMMA与经良好生产规范处理的牛骨来源的HAp（HApGMP）混合。将24个胎鼠颅骨成骨细胞培养物随机分为6组：7天和14天对照组、7天和14天PMMA - HApGMP组、7天和14天PMMA - HApBBK组。通过免疫细胞化学检查观察RUNX2和ALP的表达。
单因素方差分析的显著性值为0.000（p < 0.05）。在成骨细胞培养物中，第7天和第14天，PMMA - HApBBK组和PMMA - HApGMP组的RUNX2和ALP表达均增加。
PMMA - HApBBK和PMMA - HApGMP在成骨细胞培养物中显示出RUNX2和ALP表达增加，这表明骨植入物的骨整合可能增加。
