Saskianti Tania, Purnamasari Shinta, Pradopo Seno, Nugraha Alexander Patera, Prahasanti Chiquita, Ernawati Diah Savitri, Kanawa Masami
Department of Pediatric Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Eur J Dent. 2024 Feb;18(1):314-320. doi: 10.1055/s-0043-1768971. Epub 2023 Jun 19.
Stem cell from human exfoliated deciduous teeth (SHED) has great potential for bone tissue engineering and cell therapy for regenerative medicine. It has been combined with biomaterials such as mixed of polymethylmethacrylate (PMMA) and hydroxyapatite (HA) as candidates for synthetic bone graft biomaterial. The aim of this study was to analyze the toxicity test of mixed PMMA-HA scaffold seeded with SHED and osteoblast .
SHED was isolated from the pulp of noncarious deciduous teeth and osteoblast cells were cultured, and exposed to PMMA-HA scaffolds with three concentration groups: 20/80, 30/70, and 40/60 for 24 hours. Cytotoxicity test was performed by MTT assay to cell viability.
Data were analyzed using IBM SPSS Statistics 25, one-way analysis of variance followed by least significant difference test, considering the level of significance -value less than 0.05 RESULTS: The percentage of SHED's viability was best in the PMMA-HA group with concentrations of 20/80, followed by 30/70, and 40/60 with 87.03, 75.33, and 65.79%, respectively. The percentage of osteoblast cell's viability was best in the PMMA-HA group with concentrations of 20/80, followed by 30/70, and 40/60 with 123.6, 108.36, and 93.48%, respectively.
Mixed PMMA-HA was not toxic for the SHED and osteoblast. This characteristic is the initial requirement to be proposed as an alternative material for healing alveolar bone defects. In vivo animal research is mandatory to confirm the use of PMMA-HA on the alveolar defect model.
人脱落乳牙干细胞（SHED）在骨组织工程和再生医学的细胞治疗方面具有巨大潜力。它已与聚甲基丙烯酸甲酯（PMMA）和羟基磷灰石（HA）的混合物等生物材料结合，作为合成骨移植生物材料的候选物。本研究的目的是分析接种SHED和成骨细胞的PMMA - HA混合支架的毒性试验。
从非龋乳牙牙髓中分离出SHED并培养成骨细胞，将其暴露于三种浓度组（20/80、30/70和40/60）的PMMA - HA支架中24小时。通过MTT法对细胞活力进行细胞毒性试验。
使用IBM SPSS Statistics 25对数据进行分析，采用单因素方差分析，然后进行最小显著差异检验，显著性水平设定为p值小于0.05。结果：在浓度为20/80的PMMA - HA组中，SHED的活力百分比最高，其次是30/70组和40/60组，分别为87.03%、75.33%和65.79%。在浓度为20/80的PMMA - HA组中，成骨细胞的活力百分比最高，其次是30/70组和40/60组，分别为123.6%、108.36%和93.48%。
PMMA - HA混合物对SHED和成骨细胞无毒。这一特性是将其作为治疗牙槽骨缺损替代材料的初步要求。必须进行体内动物研究以证实PMMA - HA在牙槽骨缺损模型中的应用。
