Ma Tao, Li Jianping, Jiang Qian, Wu Sufang, Jiang Huaizhi, Zhang Qiaoling
College of Veterinary Medicine, Jilin University, Changchun, 130062, China.
College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, 132101, China.
Funct Integr Genomics. 2018 Nov;18(6):701-707. doi: 10.1007/s10142-018-0616-x. Epub 2018 Jun 18.
In order to improve the production and quality of Chinese cashmere, the research of hair follicle development has aroused more and more attention; the regulation mechanism of miRNA in hair follicle development has become a hot spot. A survey of transcriptome profiling screened 10 hair follicle-related miRNAs that were differentially expressed, including miR-let7a. In this study, the expression of miR-let7a was lower in anagen of hair follicle of cashmere goats than that in catagen of hair follicle of cashmere goats (p < 0.01). Results were in accordance with transcriptome data. The expression patterns of miR-let7a target genes (IGF-1R, C-myc, and FGF5) were verified by qRT-PCR, which were consistent with the results of Western blot and showed a downward trend. The dual-luciferase reporter gene system was used to verify the correlation between the expression of miR-let7a and its target genes, and it showed that miR-let7a negatively correlates with C-myc and FGF5. Present study offers new information on miRNAs and their related target genes in the regulation of hair follicle development mechanism.
为了提高中国羊绒的产量和质量，毛囊发育的研究引起了越来越多的关注；miRNA在毛囊发育中的调控机制已成为研究热点。一项转录组图谱调查筛选出10个与毛囊相关的差异表达miRNA，其中包括miR-let7a。本研究中，绒山羊毛囊生长期的miR-let7a表达低于退行期（p < 0.01）。结果与转录组数据一致。通过qRT-PCR验证了miR-let7a靶基因（IGF-1R、C-myc和FGF5）的表达模式，与蛋白质免疫印迹结果一致，呈下降趋势。利用双荧光素酶报告基因系统验证miR-let7a表达与其靶基因之间的相关性，结果表明miR-let7a与C-myc和FGF5呈负相关。本研究为miRNA及其相关靶基因在毛囊发育机制调控方面提供了新信息。
