Department of Neurology, Shuang Ho Hospital, Taipei Medical University, Taipei, Taiwan.
Department of Neurology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
Aging (Albany NY). 2023 Mar 9;15(5):1603-1614. doi: 10.18632/aging.204575.
Inflammation contributes substantially to the pathogenesis of Parkinson's disease (PD). Plasma extracellular vesicle (EV)-derived cytokines are emerging biomarkers of inflammation. We conducted a longitudinal study of the plasma EV-derived cytokine profiles of people with PD (PwP).
A total of 101 people with mild to moderate PD and 45 healthy controls (HCs) were recruited, and they completed motor assessments (Unified Parkinson Disease Rating Scale [UPDRS]) and cognitive tests at baseline and 1-year follow-up. We isolated the participants' plasma EVs and analyzed their levels of cytokines, including interleukin (IL)-1β, IL-6, IL-10, tumor necrosis factor (TNF)-α, and transforming growth factor (TGF)-β.
We noted no significant changes in the plasma EV-derived cytokine profiles of the PwPs and HCs between baseline and the 1-year follow-up. Among the PwP, changes in plasma EV-derived IL-1β, TNF-α and IL-6 levels were significantly associated with changes in the severity of postural instability and gait disturbance (PIGD) and cognition. Baseline plasma EV-derived IL-1β, TNF-α, IL-6, and IL-10 levels were significantly associated with the severity of PIGD and cognitive symptoms at follow-up, and PwP with elevated IL-1β and IL-6 levels exhibited significant progression of PIGD over the study period.
These results suggested the role of inflammation in PD progression. In addition, baseline levels of plasma EV-derived proinflammatory cytokines can be used to predict the progression of PIGD, the most severe motor symptom of PD. Additional studies with longer follow-up periods are necessary, and plasma EV-derived cytokines may serve as effective biomarkers of PD progression.
炎症在帕金森病（PD）的发病机制中起重要作用。血浆细胞外囊泡（EV）衍生细胞因子是炎症的新兴生物标志物。我们对 PD 患者（PwP）的血浆 EV 衍生细胞因子谱进行了纵向研究。
共招募了 101 名轻度至中度 PD 患者和 45 名健康对照者（HCs），他们在基线和 1 年随访时完成了运动评估（统一帕金森病评定量表[UPDRS]）和认知测试。我们分离了参与者的血浆 EV，并分析了细胞因子的水平，包括白细胞介素（IL）-1β、IL-6、IL-10、肿瘤坏死因子（TNF）-α和转化生长因子（TGF）-β。
我们没有注意到 PwP 和 HCs 的血浆 EV 衍生细胞因子谱在基线和 1 年随访之间有显著变化。在 PwP 中，血浆 EV 衍生的 IL-1β、TNF-α 和 IL-6 水平的变化与姿势不稳和步态障碍（PIGD）和认知的严重程度变化显著相关。基线血浆 EV 衍生的 IL-1β、TNF-α、IL-6 和 IL-10 水平与随访时 PIGD 和认知症状的严重程度显著相关，且 IL-1β 和 IL-6 水平升高的 PwP 在研究期间 PIGD 显著进展。
这些结果表明炎症在 PD 进展中的作用。此外，基线血浆 EV 衍生促炎细胞因子水平可用于预测 PIGD 的进展，PIGD 是 PD 最严重的运动症状。需要进行更长随访期的进一步研究，并且血浆 EV 衍生细胞因子可能是 PD 进展的有效生物标志物。
