Diaz K, Kohut M L, Russell D W, Stegemöller E L
Department of Kinesiology, Iowa State University, Ames, IA, USA.
Department of Human Development & Family Studies, Iowa State University, Ames, IA, USA.
Brain Behav Immun Health. 2022 Mar 7;21:100442. doi: 10.1016/j.bbih.2022.100442. eCollection 2022 May.
Many of the motor symptoms of Parkinson's disease (PD) impact quality of life and are not fully ameliorated by current pharmacological and surgical treatments. A better understanding of the pathophysiology underlying these symptoms is needed. Previous research has suggested that inflammation may play a significant role in PD pathophysiology and progression, but there is limited research exploring how inflammation directly relates to motor symptoms in PD. Thus, the purpose of this study was to evaluate associations between peripheral immune inflammatory markers and motor symptoms of PD, specifically, tremor, bradykinesia, and postural and gait instability. We hypothesized that peripheral inflammatory cytokines would predict the severity of motor symptoms in persons with PD, and that there will be higher levels of peripheral inflammatory cytokine markers in persons with PD when compared to age-matched healthy older adults.
Twenty-six participants with PD and fourteen healthy older adults completed the study. For participants with PD, the motor section of the Unified Parkinson's Disease Rating Scale (UPDRS) was recorded and scored by two Movement Disorders Neurologists masked to the study. A blood sample was collected from both participants with PD and the healthy older adults. Through the MILLIPLEX® map High Sensitivity Human Cytokine Kit, key inflammation-related markers were analyzed (TNF-, IFN-γ, IL-1β, IL-8, IL-2, IL-7, IL-5, IL-13, IL, 4, IL-10 IL-12p70, GM-CSF, and IL-6).
Results revealed significantly higher levels of IL-6 in persons with PD when compared to healthy older adults ( = 0.005). Moreover, results revealed that higher levels of IL-4 ( = 0.011) and lower levels of IFNγ ( = 0.003) significantly predicted more severe tremor in persons with PD. No other associations between the peripheral inflammation markers and other motor symptoms were observed.
Overall, these results are consistent with a growing body of literature that implicates inflammatory cytokines in the PD, and further suggests that inflammatory cytokines, or lack thereof, may be associated with tremor in persons with PD.
帕金森病（PD）的许多运动症状会影响生活质量，并且目前的药物和手术治疗并不能完全改善这些症状。需要更好地了解这些症状背后的病理生理学。先前的研究表明，炎症可能在PD的病理生理学和进展中起重要作用，但探索炎症与PD运动症状直接关系的研究有限。因此，本研究的目的是评估外周免疫炎症标志物与PD运动症状之间的关联，特别是震颤、运动迟缓以及姿势和步态不稳。我们假设外周炎症细胞因子可以预测PD患者运动症状的严重程度，并且与年龄匹配的健康老年人相比，PD患者外周炎症细胞因子标志物水平会更高。
26名PD患者和14名健康老年人完成了该研究。对于PD患者，由两名对研究不知情的运动障碍神经科医生记录并评分统一帕金森病评定量表（UPDRS）的运动部分。从PD患者和健康老年人中均采集了血样。通过MILLIPLEX®图谱高灵敏度人细胞因子试剂盒，分析了关键的炎症相关标志物（肿瘤坏死因子-α、干扰素-γ、白细胞介素-1β、白细胞介素-8、白细胞介素-2、白细胞介素-7、白细胞介素-5、白细胞介素-13、白细胞介素-4、白细胞介素-10、白细胞介素-12p70、粒细胞巨噬细胞集落刺激因子和白细胞介素-6）。
结果显示，与健康老年人相比，PD患者的白细胞介素-6水平显著更高（P = 0.005）。此外，结果表明，较高水平的白细胞介素-4（P = 0.011）和较低水平的干扰素-γ（P = 0.003）显著预测了PD患者更严重的震颤。未观察到外周炎症标志物与其他运动症状之间的其他关联。
总体而言，这些结果与越来越多的将炎症细胞因子与PD相关联的文献一致，并且进一步表明炎症细胞因子或其缺乏可能与PD患者的震颤有关。
