Research Center for Translational Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200092, China.
Research Center for Translational Medicine, Shanghai East Hospital, GuiLin University School of Medicine, Guilin, 541004, China.
Hum Cell. 2023 May;36(3):1099-1107. doi: 10.1007/s13577-023-00884-9. Epub 2023 Mar 10.
Eukaryotic translation initiation factor 4 gamma 1 (EIF4G1) is highly expressed in many cancers and affects their occurrence and development. However, the effect of EIF4G1 on the prognosis, biological function and the relevant mechanism in lung squamous cell carcinoma (LSCC) is unclear. Through clinical cases, Cox's proportional hazard model and Kaplan-Meier plotter survival analysis, we find the expression levels of EIF4G1 are dependent on age and clinical stage, high expression of EIF4G1 could be used to predict the overall survival of LSCC patients. LSCC cell line NCI-H1703, NCI-H226 and SK-MES-1infected with EIF4G1 siRNA are used to detect the function of EIF4G1 with cell proliferation and tumorigenesis in vivo and vitro. The data show that EIF4G1 promotes tumor cell proliferation and the G1/S transition of cell cycle in LSCC, then the biological function of LSCC is effected by the AKT/mTOR pathway. Above all, these results have demonstrated that EIF4G1 promotes LSCC cell proliferation and may represent an indicator of prognosis in LSCC.
真核翻译起始因子 4 γ 1（EIF4G1）在许多癌症中高度表达，影响其发生和发展。然而，EIF4G1 对肺鳞状细胞癌（LSCC）的预后、生物学功能及相关机制的影响尚不清楚。通过临床病例、Cox 比例风险模型和 Kaplan-Meier plotter 生存分析，我们发现 EIF4G1 的表达水平与年龄和临床分期相关，EIF4G1 的高表达可用于预测 LSCC 患者的总生存率。用 EIF4G1 siRNA 感染 LSCC 细胞系 NCI-H1703、NCI-H226 和 SK-MES-1，以检测 EIF4G1 在体外和体内对肿瘤细胞增殖和致瘤性的功能。结果表明，EIF4G1 促进 LSCC 中的肿瘤细胞增殖和细胞周期的 G1/S 期转变，从而影响 LSCC 的生物学功能，AKT/mTOR 通路在此过程中发挥作用。综上所述，这些结果表明 EIF4G1 促进 LSCC 细胞增殖，可能是 LSCC 预后的一个指标。
