Turco S J, Hull S R, Orlandi P A, Shepherd S D, Homans S W, Dwek R A, Rademacher T W
Department of Biochemistry, University of Kentucky Medical Center, Lexington 40536.
Biochemistry. 1987 Sep 22;26(19):6233-8. doi: 10.1021/bi00393a042.
The major carbohydrate fragment from the lipophosphoglycan of Leishmania donovani was generated by mild acid hydrolysis (0.02 N HCl, 5 min, 100 degrees C) and purified by chromatography on DE-52 cellulose and thin layer. By a combination of analyses including gas-liquid chromatography-mass spectrometry and 1H NMR, the structure of the fragment was elucidated as PO4----6Gal(beta 1----4)Man. Approximately 16 of these phosphorylated disaccharide units occur in the overall glycoconjugate structure. NMR analysis of an alkaline phosphatase treated phosphorylated tetrasaccharide generated from lipophosphoglycan showed that the phosphorylated disaccharide units are linked together via alpha-glycosidic linkages. Complete characterization of the phosphorylated disaccharide units of lipophosphoglycan provides the first example of a defined carbohydrate anchored in membranes by a derivative of phosphatidylinositol.
杜氏利什曼原虫脂磷酸聚糖的主要碳水化合物片段通过温和酸水解（0.02N盐酸，5分钟，100℃）产生，并通过DE-52纤维素柱色谱和薄层色谱进行纯化。通过气-液色谱-质谱联用和1H NMR等多种分析方法，该片段的结构被阐明为PO4----6Gal(β1----4)Man。在整个糖缀合物结构中大约存在16个这种磷酸化二糖单元。对脂磷酸聚糖产生的碱性磷酸酶处理的磷酸化四糖进行NMR分析表明，磷酸化二糖单元通过α-糖苷键连接在一起。脂磷酸聚糖磷酸化二糖单元的完整表征提供了第一个由磷脂酰肌醇衍生物锚定在膜上的特定碳水化合物的实例。
