Aitken Robert J, Wilkins Alexandra, Harrison Natasha, Bahrami Mohammad, Gibb Zamira, McIntosh Kaitlin, Vuong Quan, Lambourne Sarah
Centre for Reproductive Science, University of Newcastle, Newcastle, NSW 2308, Australia.
Hunter Medical Research Institute, Newcastle, NSW 2305, Australia.
Antioxidants (Basel). 2025 Jan 14;14(1):90. doi: 10.3390/antiox14010090.
(1) Background: The RoXsta system has been developed as a rapid, effective means of profiling different types of antioxidant activity. The purpose of this study was to examine its performance utilizing a diverse array of biological fluids including semen, blood plasma, serum, urine, saliva, follicular fluid and plant extracts. (2) Methods: The RoXsta system was used to assess the ability of different fluids to suppress free radical formation as well as scavenge a variety of toxic oxygen metabolites including free radicals and both hydrogen and organic peroxides. (3) Results: Human semen was shown to have significantly ( < 0.001) more peroxide scavenging power than any other fluid tested (10-14 mM vitamin C equivalent compared with 1-2 mM for blood serum or plasma), while urine was particularly effective in scavenging free radicals and preventing free radical formation ( < 0.001). The powerful antioxidant properties of human semen were shown to reside within the seminal plasma (SP) fraction, rather than the spermatozoa, and to be resistant to snap freezing in liquid nitrogen. Moreover, comparative studies demonstrated that human SP exhibited significantly ( < 0.001) higher levels of antioxidant potential than any other species examined (stallion, bull, dog) and that this intense activity reflected the relative vulnerability of human spermatozoa to peroxide attack. (4) Conclusions: The RoXsta system provides valuable information on the antioxidant profile of complex biological fluids, supporting its diagnostic role in conditions associated with oxidative stress. Based on the results secured in this study, human semen is identified as a particularly rich source of antioxidants capable of scavenging both hydrogen and organic peroxides, in keeping with the high susceptibility of human spermatozoa to peroxide-mediated damage.
(1) 背景：RoXsta系统已被开发成为一种快速、有效的分析不同类型抗氧化活性的方法。本研究的目的是利用包括精液、血浆、血清、尿液、唾液、卵泡液和植物提取物在内的多种生物流体来检验其性能。(2) 方法：使用RoXsta系统评估不同流体抑制自由基形成以及清除多种有毒氧代谢物（包括自由基、过氧化氢和有机过氧化物）的能力。(3) 结果：研究表明，人类精液清除过氧化物的能力显著更强（<0.001），高于任何其他测试流体（相当于10 - 14 mM维生素C，而血清或血浆为1 - 2 mM），而尿液在清除自由基和防止自由基形成方面特别有效（<0.001）。人类精液强大的抗氧化特性存在于精浆（SP）部分，而非精子中，并且对液氮速冻具有抗性。此外，比较研究表明，人类SP的抗氧化潜力水平显著高于所检测的任何其他物种（种马、公牛、狗）（<0.001），这种强烈的活性反映了人类精子对过氧化物攻击的相对脆弱性。(4) 结论：RoXsta系统提供了关于复杂生物流体抗氧化谱的有价值信息，支持其在与氧化应激相关病症中的诊断作用。基于本研究获得的结果，人类精液被确定为一种特别丰富的抗氧化剂来源，能够清除过氧化氢和有机过氧化物，这与人类精子对过氧化物介导损伤的高度敏感性一致。
