Harant Karel, Čajka Tomáš, Angelisová Pavla, Pokorná Jana, Hořejší Václav
Proteomics Core Facility, Faculty of Science, Charles University, BIOCEV, Prumyslova 595, Vestec CZ-25242, Czechia; Institute for Environmental Studies, Faculty of Science, Charles University, Benatska 2, Prague 2 CZ-128 01, Czechia.
Institute of Physiology of the Czech Academy of Sciences, Vídeňská, 1083 142 20 Praha 4, Czechia.
Biophys Chem. 2023 May;296:106989. doi: 10.1016/j.bpc.2023.106989. Epub 2023 Mar 5.
An advantageous alternative to the use of detergents in biochemical studies on membrane proteins are the recently developed styrene-maleic acid (SMA) amphipathic copolymers. In our recent study we demonstrated that using this approach, most T cell membrane proteins were fully solubilized (presumably in small nanodiscs), while two types of raft proteins, GPI-anchored proteins and Src family kinases, were mostly present in much larger (>250 nm) membrane fragments markedly enriched in typical raft lipids, cholesterol and lipids containing saturated fatty acid residues. In the present study we demonstrate that disintegration of membranes of several other cell types by means of SMA copolymer follows a similar pattern and we provide a detailed proteomic and lipidomic characterization of these SMA-resistant membrane fragments (SRMs).
在膜蛋白的生化研究中，一种优于使用去污剂的方法是最近开发的苯乙烯-马来酸（SMA）两亲共聚物。在我们最近的研究中，我们证明使用这种方法，大多数T细胞膜蛋白被完全溶解（可能形成小的纳米盘），而两种类型的脂筏蛋白，糖基磷脂酰肌醇（GPI）锚定蛋白和Src家族激酶，大多存在于明显富集典型脂筏脂质、胆固醇和含有饱和脂肪酸残基的脂质的大得多（>250 nm）的膜碎片中。在本研究中，我们证明通过SMA共聚物使其他几种细胞类型的膜解体遵循类似的模式，并且我们提供了这些抗SMA膜碎片（SRM）的详细蛋白质组学和脂质组学特征。
