Xu Yonghua, Meng Xianghui, Song Yunong, Lv Xiaoyi, Sun Yong
Northeast Agricultural University, Harbin 150030, China; Key Laboratory of Agricultural Renewable Resources Utilization Technology and Equipment in Cold Areas of Heilongjiang Province, Harbin, 150030, China; Key Laboratory of Pig-breeding Facilities Engineering, Ministry of Agriculture, Harbin 150030, China.
Northeast Agricultural University, Harbin 150030, China; Key Laboratory of Agricultural Renewable Resources Utilization Technology and Equipment in Cold Areas of Heilongjiang Province, Harbin, 150030, China; Key Laboratory of Pig-breeding Facilities Engineering, Ministry of Agriculture, Harbin 150030, China.
Bioresour Technol. 2023 Jun;377:128845. doi: 10.1016/j.biortech.2023.128845. Epub 2023 Mar 9.
Investigating the effect of butyric acid concentration on anaerobic digestion systems in complex systems is important for the efficient degradation of butyric acid and improving the efficiency of anaerobic digestion. In this study, different loadings of butyric acid with 2.8, 3.2, and 3.6 g/(L·d) were added to the anaerobic reactor. At a high organic loading rate of 3.6 g/(L·d), methane was efficiently produced with VBP (Volumetric Biogas Production) of 1.50 L/(L·d) and biogas content between 65% and 75%. VFAs concentration remained below 2000 mg/L. Metagenome sequencing revealed changes in the functional flora within different stages. Methanosarcina, Syntrophomonas, and Lentimicrobium were the main and functional microorganisms. That the relative abundance of methanogens exceeded 35% and methanogenic metabolic pathways were increased indicated the methanogenic capacity of the system significantly improved. The presence of a large number of hydrolytic acid-producing bacteria also indicated the importance of the hydrolytic acid-producing stage in the system.
研究丁酸浓度对复杂系统中厌氧消化系统的影响，对于丁酸的高效降解和提高厌氧消化效率具有重要意义。在本研究中，向厌氧反应器中添加了不同负荷的丁酸，分别为2.8、3.2和3.6 g/(L·d)。在3.6 g/(L·d)的高有机负荷率下，高效产生了甲烷，体积沼气产量（VBP）为1.50 L/(L·d)，沼气含量在65%至75%之间。挥发性脂肪酸（VFA）浓度保持在2000 mg/L以下。宏基因组测序揭示了不同阶段功能菌群的变化。甲烷八叠球菌、互营单胞菌和慢微菌是主要的功能微生物。产甲烷菌的相对丰度超过35%且产甲烷代谢途径增加，表明系统的产甲烷能力显著提高。大量水解产酸细菌的存在也表明了水解产酸阶段在系统中的重要性。
