Tuersong Waresi, Liu Xin, Wang Yifan, Wu Simin, Qin Peixi, Zhu Shengnang, Liu Feng, Wang Chunqun, Hu Min
State Key Laboratory of Agricultural Microbiology, Key Laboratory of Development of Veterinary Products, Ministry of Agriculture, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Animals (Basel). 2023 Jan 28;13(3):456. doi: 10.3390/ani13030456.
Resistance to anthelmintics such as ivermectin (IVM) is currently a major problem in the treatment of , an important parasitic nematode of small ruminants. Although many advances have been made in understanding the IVM resistance mechanism, its exact mechanism remains unclear for . Therefore, understanding the resistance mechanism becomes increasingly important for controlling haemonchosis. Recent research showed that the metabolic state of bacteria influences their susceptibility to antibiotics. However, little information is available on the roles of metabolites and metabolic pathways in IVM resistance of . In this study, comparative analyses of the metabolomics of IVM-susceptible and -resistant adult worms were carried out to explore the role of metabolism in IVM resistance. In total, 705 metabolites belonging to 42 categories were detected, and 86 differential metabolites (17 upregulated and 69 downregulated) were identified in the IVM-resistant strain compared to the susceptible one. A KEGG pathway analysis showed that these 86 differential metabolites were enriched in 42 pathways that mainly included purine metabolism; the biosynthesis of amino acids; glycine, serine, and threonine metabolism; and cysteine and methionine metabolism. These results showed that amino acid metabolism may be mediated by the uptake of IVM and related with IVM resistance in This study contributes to our understanding of the mechanisms of IVM resistance and may provide effective approaches to manage infection by resistant strains of .
对伊维菌素（IVM）等驱虫药产生抗性目前是治疗小型反刍动物重要寄生线虫捻转血矛线虫的一个主要问题。尽管在理解伊维菌素抗性机制方面已取得许多进展，但其确切机制对于捻转血矛线虫仍不清楚。因此，了解抗性机制对于控制血矛线虫病变得越来越重要。最近的研究表明，细菌的代谢状态会影响它们对抗生素的敏感性。然而，关于代谢物和代谢途径在捻转血矛线虫伊维菌素抗性中的作用，目前所知甚少。在本研究中，对伊维菌素敏感和抗性成虫捻转血矛线虫进行了代谢组学比较分析，以探索捻转血矛线虫代谢在伊维菌素抗性中的作用。总共检测到属于42类的705种代谢物，与敏感品系相比，在伊维菌素抗性品系中鉴定出86种差异代谢物（17种上调和69种下调）。KEGG通路分析表明，这86种差异代谢物富集在42条通路中，主要包括嘌呤代谢；氨基酸的生物合成；甘氨酸、丝氨酸和苏氨酸代谢；以及半胱氨酸和甲硫氨酸代谢。这些结果表明，氨基酸代谢可能由伊维菌素的摄取介导，并与捻转血矛线虫的伊维菌素抗性相关。本研究有助于我们理解伊维菌素抗性机制，并可能为管理捻转血矛线虫抗性菌株感染提供有效方法。
