Gao Xiaodong, Wang Xibao, Wu Xiaoyang, Shang Yongquan, Mei Xuesong, Zhou Shengyang, Wei Qinguo, Sun Guolei, Dong Yuehuan, Cui Weijia, Zhang Honghai
College of Life Sciences, Qufu Normal University, Qufu 273165, China.
Animals (Basel). 2023 Mar 3;13(5):923. doi: 10.3390/ani13050923.
Black-billed capercaillie () was listed as a first-class state-protected animal because it was endangered in China (Category I). This study is the first to examine the diversity and composition of gut microbiome in the wild. We collected fecal samples from five black-billed capercaillie flock roosting sites (each 20 km apart) in one day. Thirty fecal samples were sequenced with 16S rRNA gene amplicons on the Illumina HiSeq platform. This study is the first to analyze the fecal microbiome composition and diversity of black-billed capercaillie in the wild. At the phylum level, Camplyobacterota, Bacillota, Cyanobacteria, Actinomycetota, and Bacteroidota were the most abundant in the fecal microbiome of black-billed capercaillie. At the genus level, , , , , and were the dominant genera. Based on alpha and beta diversity analyses, we found no significant differences in the fecal microbiome between five flocks of black-billed capercaillie. Protein families: genetic information processing; protein families: signaling and cellular processes, carbohydrate metabolism; protein families: metabolism and energy metabolism are the main predicted functions of the black-billed capercaillie gut microbiome through the PICRUSt2 method. This study reveals the composition and structure of the fecal microbiome of the black-billed capercaillie under wild survival conditions, and this study provides scientific data for the comprehensive conservation of the black-billed capercaillie.
黑嘴松鸡（）被列为国家一级保护动物，因为它在中国处于濒危状态（一级）。本研究首次对野生黑嘴松鸡肠道微生物群的多样性和组成进行了研究。我们在一天内从五个黑嘴松鸡群的栖息地（彼此相距20公里）采集了粪便样本。在Illumina HiSeq平台上对30个粪便样本进行了16S rRNA基因扩增子测序。本研究首次分析了野生黑嘴松鸡的粪便微生物群组成和多样性。在门水平上，弯曲杆菌门、芽孢杆菌门、蓝细菌门、放线菌门和拟杆菌门在黑嘴松鸡粪便微生物群中最为丰富。在属水平上， 、 、 、 和 是优势属。基于α和β多样性分析，我们发现五群黑嘴松鸡的粪便微生物群之间没有显著差异。蛋白质家族：遗传信息处理；蛋白质家族：信号传导和细胞过程、碳水化合物代谢；蛋白质家族：代谢和能量代谢是通过PICRUSt2方法预测的黑嘴松鸡肠道微生物群的主要功能。本研究揭示了野生生存条件下黑嘴松鸡粪便微生物群的组成和结构，为黑嘴松鸡的全面保护提供了科学数据。
