Universidade Federal do Rio Grande do Sul (UFRGS), Faculdade de Veterinária, Setor de Suínos, Porto Alegre, RS, Brazil.
Institute for Reproduction of Farm Animals Schönow (IFN), Bernau, Germany.
Anim Reprod Sci. 2023 Aug;255:107293. doi: 10.1016/j.anireprosci.2023.107293. Epub 2023 Jul 1.
This study evaluated the effect of sperm concentration of boar semen doses on their capacity to maintain its motility over the thermo-resistance test (TRT; sperm resilience) and verified if the extender type (short or long-term) could influence this effect. Thirty ejaculates from five crossbred mature PIC® boars were used, and a factorial design was followed to produce semen doses with 1.5 billion cells in 45 or 90 mL, using Beltsville Thawing Solution (BTS) or Androstar® Plus (APlus). Then, low-concentration doses (16.7 × 10 cells/mL in 90 mL) and higher-concentration doses (33.3 × 10 cells/mL in 45 mL) with BTS or APlus were produced and stored at 17 °C for 168 h. At 72 h, during the TRT, the low-concentration doses (16.7 × 10 cells/mL) lost three-fold less motility than doses with 33.3 × 10 cells/mL (P < 0.01), regardless of the extender type (11. 5% vs. 30.5% of initial motility, respectively). Similar results were found when the TRT was carried out at 168 h, with low-concentration doses losing two-fold less motility (11.4%) than highly concentrated doses (25.9%; P < 0.01). No sperm concentration effect was observed on membrane integrity or mitochondrial membrane potential (P ≥ 0.23). The osmolarity was not affected by the sperm concentration (P = 0.56), only by the extender and the storage time (P < 0.01). In conclusion, the sperm concentration effect on sperm quality was not influenced by extender type, and the data suggest that a low concentration of semen doses positively affects sperm resilience.
本研究评估了猪精液精子浓度对其在耐热试验（TRT；精子弹性）中保持活力的能力的影响，并验证了 extender 类型（短期或长期）是否会影响这种效应。使用来自 5 头杂交成熟 PIC®公猪的 30 个精液样本，采用析因设计生产 45 或 90ml 中含有 15 亿个精子的精液剂量，使用 Beltsville Thawing Solution（BTS）或 Androstar® Plus（APlus）。然后，使用 BTS 或 APlus 生产低浓度剂量（90ml 中 16.7×10 个细胞/ml）和高浓度剂量（45ml 中 33.3×10 个细胞/ml），并在 17°C 下储存 168 小时。在 TRT 期间的 72 小时，低浓度剂量（16.7×10 个细胞/ml）的活力损失比 33.3×10 个细胞/ml 的剂量低三倍（P<0.01），与 extender 类型无关（分别为初始活力的 11.5%和 30.5%）。当在 168 小时进行 TRT 时，也得到了类似的结果，低浓度剂量的活力损失比高浓度剂量少两倍（11.4%比 25.9%；P<0.01）。精子浓度对膜完整性或线粒体膜电位没有影响（P≥0.23）。渗透压不受精子浓度的影响（P=0.56），仅受 extender 和储存时间的影响（P<0.01）。总之，精子浓度对精子质量的影响不受 extender 类型的影响，并且数据表明低浓度的精液剂量对精子弹性有积极影响。
