Liu Jing-Hong, Zheng Yu-Yun, Ouyang Jian-Ming
Institute of Biomineralization and Lithiasis Research, College of Chemistry and Materials Science, Jinan University, Guangzhou 510632, China.
Foods. 2023 Feb 28;12(5):1031. doi: 10.3390/foods12051031.
The antioxidant activities of seven degraded products (GLPs) with different molecular weights () of polysaccharides from were compared. The of GLP1-GLP7 were 106, 49.6, 10.5, 6.14, 5.06, 3.71 and 2.42 kDa, respectively. The results show that GLP2 with = 49.6 kDa had the strongest scavenging capacity for hydroxyl radical, DPPH radical, ABTS radical and reducing power. When < 49.6 kDa, the antioxidant activity of GLPs increased with the increase in , but when increased to 106 kDa, their antioxidant activity decreased. However, the ability of GLPs to chelate Fe ions increased with the decrease in polysaccharide , which was attributed to the fact that the polysaccharide active groups (-OSO and -COOH) were easier to expose, and the steric hindrance was smaller when GLPs chelated with Fe. The effects of GLP1, GLP3, GLP5 and GLP7 on the crystal growth of calcium oxalate (CaOx) were studied using XRD, FT-IR, Zeta potential and thermogravimetric analysis. Four kinds of GLPs could inhibit the growth of calcium oxalate monohydrate (COM) and induce the formation of calcium oxalate dihydrate (COD) in varying degrees. With the decrease in of GLPs, the percentage of COD increased. GLPs increased the absolute value of the Zeta potential on the crystal surface and reduced the aggregation between crystals. Cell experiments showed that the toxicity of CaOx crystal regulated by GLPs to HK-2 cells was reduced, and the cytotoxicity of CaOx crystal regulated by GLP7 with the smallest was the smallest, which was consistent with the highest SOD activity, the lowest ROS and MDA levels, the lowest OPN expression level and the lowest cell necrosis rate. These results suggest that GLPs, especially GLP7, may be a potential drug for the prevention and treatment of kidney stones.
比较了从[具体来源未给出]提取的具有不同分子量（）的七种降解产物（GLPs）的抗氧化活性。GLP1 - GLP7的分子量分别为106、49.6、10.5、6.14、5.06、3.71和2.42 kDa。结果表明，分子量为49.6 kDa的GLP2对羟基自由基、DPPH自由基、ABTS自由基的清除能力和还原力最强。当分子量小于49.6 kDa时，GLPs的抗氧化活性随分子量的增加而增强，但当分子量增加到106 kDa时，其抗氧化活性降低。然而，GLPs螯合铁离子的能力随多糖分子量的降低而增强，这是由于多糖活性基团（-OSO和-COOH）更容易暴露，且GLPs与铁螯合时空间位阻较小。利用XRD、FT - IR、Zeta电位和热重分析研究了GLP1、GLP3、GLP5和GLP7对草酸钙（CaOx）晶体生长的影响。四种GLPs均可不同程度地抑制一水草酸钙（COM）的生长并诱导二水草酸钙（COD）的形成。随着GLPs分子量的降低，COD的百分比增加。GLPs增加了晶体表面Zeta电位的绝对值，减少了晶体间的聚集。细胞实验表明，GLPs调节的CaOx晶体对HK - 2细胞的毒性降低，分子量最小的GLP7调节的CaOx晶体的细胞毒性最小，这与最高的超氧化物歧化酶（SOD）活性、最低的活性氧（ROS）和丙二醛（MDA）水平、最低的骨桥蛋白（OPN）表达水平以及最低的细胞坏死率一致。这些结果表明，GLPs，尤其是GLP7，可能是预防和治疗肾结石的潜在药物。
