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5-氨基酮戊酸的光动力效应对克罗恩病患者外周血单个核细胞细胞因子和外泌体的影响。

Photodynamic Effects with 5-Aminolevulinic Acid on Cytokines and Exosomes in Human Peripheral Blood Mononuclear Cells from Patients with Crohn's Disease.

机构信息

Department of Gastroenterology, Akershus University Hospital, 1478 Lorenskog, Norway.

Department of Pathology, Norwegian Radium Hospital, Oslo University Hospital, 0310 Oslo, Norway.

出版信息

Int J Mol Sci. 2023 Feb 25;24(5):4554. doi: 10.3390/ijms24054554.

DOI:10.3390/ijms24054554
PMID:36901982
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10003466/
Abstract

Photodynamic therapy (PDT) using 5-aminolevulinic acid (ALA) which is the precursor of the photosensitizer protoporphyrin IX (PpIX) is an available treatment for several diseases. ALA-PDT induces the apoptosis and necrosis of target lesions. We have recently reported the effects of ALA-PDT on cytokines and exosomes of human healthy peripheral blood mononuclear cells (PBMCs). This study has investigated the ALA-PDT-mediated effects on PBMC subsets from patients with active Crohn's disease (CD). No effects on lymphocyte survival after ALA-PDT were observed, although the survival of CD3/CD19 B-cells seemed slightly reduced in some samples. Interestingly, ALA-PDT clearly killed monocytes. The subcellular levels of cytokines and exosomes associated with inflammation were widely downregulated, which is consistent with our previous findings in PBMCs from healthy human subjects. These results suggest that ALA-PDT may be a potential treatment candidate for CD and other immune-mediated diseases.

摘要

光动力疗法（PDT）使用 5-氨基酮戊酸（ALA）作为光敏剂原卟啉 IX（PpIX）的前体，是治疗多种疾病的有效方法。ALA-PDT 诱导靶病变的细胞凋亡和坏死。我们最近报道了 ALA-PDT 对人健康外周血单个核细胞（PBMC）细胞因子和外泌体的影响。本研究探讨了 ALA-PDT 对活动期克罗恩病（CD）患者 PBMC 亚群的影响。尽管在一些样本中 CD3/CD19 B 细胞的存活似乎略有降低，但未观察到 ALA-PDT 后淋巴细胞存活的影响。有趣的是，ALA-PDT 可明显杀死单核细胞。与炎症相关的细胞因子和外泌体的亚细胞水平广泛下调，这与我们之前在健康人类 PBMC 中的发现一致。这些结果表明，ALA-PDT 可能是治疗 CD 和其他免疫介导性疾病的潜在候选方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8479/10003466/b5594cf25854/ijms-24-04554-g010.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8479/10003466/0c7c26b01aff/ijms-24-04554-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8479/10003466/b5594cf25854/ijms-24-04554-g010.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8479/10003466/08a67d886361/ijms-24-04554-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8479/10003466/bb2bef4e4a03/ijms-24-04554-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8479/10003466/6e3e0fcd5d74/ijms-24-04554-g008.jpg
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