健康和癌症三维（3D）乳腺细胞培养中的细胞 H MR 弛豫时间。
Cellular H MR Relaxation Times in Healthy and Cancer Three-Dimensional (3D) Breast Cell Culture.
机构信息
Department of Photomedicine and Physical Chemistry, Medical College, Rzeszów University, 35-310 Rzeszów, Poland.
Department of Biochemistry and General Chemistry, Medical College, Rzeszów University, 35-310 Rzeszów, Poland.
出版信息
Int J Mol Sci. 2023 Mar 1;24(5):4735. doi: 10.3390/ijms24054735.
Noninvasive measurements of H Magnetic Resonance Imaging (MR) relaxation times in a three-dimensional (3D) cell culture construct are presented. Trastuzumab was used as a pharmacological component delivered to the cells in vitro. The purpose of this study was to evaluate the Trastuzumab delivery by relaxation times in 3D cell cultures. The bioreactor has been designed and used for 3D cell cultures. Four bioreactors were prepared, two with normal cells and two with breast cancer cells. The relaxation times of HTB-125 and CRL 2314 cell cultures were determined. An immunohistochemistry (IHC) test was performed before MRI measurements to confirm the amount of HER2 protein in the CRL-2314 cancer cells. The results showed that the relaxation time of CRL2314 cells is lower than normal HTB-125 cells in both cases, before and after treatment. An analysis of the results showed that 3D culture studies have potential in evaluating treatment efficacy using relaxation times measurements with a field of 1.5 Tesla. The use H MRI relaxation times allows for the visualization of cell viability in response to treatment.
介绍了一种在三维（3D）细胞培养结构中对 H 磁共振成像（MR）弛豫时间进行非侵入性测量的方法。曲妥珠单抗被用作体外递送至细胞的药理学成分。本研究的目的是通过 3D 细胞培养中的弛豫时间来评估曲妥珠单抗的递送。已经设计并使用生物反应器进行 3D 细胞培养。准备了四个生物反应器，两个用于正常细胞，两个用于乳腺癌细胞。测定了 HTB-125 和 CRL 2314 细胞培养物的弛豫时间。在 MRI 测量之前进行了免疫组织化学（IHC）测试，以确认 CRL-2314 癌细胞中 HER2 蛋白的含量。结果表明，在治疗前后，CRL2314 细胞的弛豫时间均低于正常 HTB-125 细胞。结果分析表明，使用 1.5 特斯拉场的弛豫时间测量进行 3D 培养研究具有评估治疗效果的潜力。H MRI 弛豫时间的使用允许可视化细胞对治疗的存活能力。
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